Impact of sevoflurane and high concentration of oxygen on expression of caspase-3 and cognition of aged mice

doi:10.3969/j.issn.1006-7795.2017.03.004

Abstract

Abstract: Objective To investigate the impact of sevoflurane and high concentration of oxygen on expression of caspase-3 and cognitive function of aged mice. Methods Thirty three healthy male C57/BL6 elderly mice (14 months) were randomly divided into three groups:group of high concentration of oxygen (HO group, n=11):inhaling 100% oxygen for 4 h; high concentration of oxygen + sevoflurane group (HS group, n=11):taking in 100% oxygen with 2.5% sevoflurane for 4 h; control group (Con group, n=11):taking in air for 4 h. Cognitive function in the elderly mice was tested in the Morris water maze. Western blotting is used to test the expression of caspase-3 in hippocampus. Results Compared with Group Con, Group HO saw no difference in numbers of crossing the platform and Caspase-3 expression. Group HS crossed the platform less (P=0.023),and the caspase-3 expression of the group increased significantly(P=0.036).Conclusion Inhaling 100% oxygen had no impact on cognitive function in the elderly mice;Inhaling 2.5% Sevoflurane with 100% oxygen could increase expression of caspase-3, and it had close relations with the cognitive dysfunction of the aged mice.

Key words: elderly mice, cognitive dysfunction, sevoflurane, oxygen, caspase

CLC Number:

R614

Chen Bin, Liu Wei, Shi Zhiguo, Liu Tao, Zheng Hui. Impact of sevoflurane and high concentration of oxygen on expression of caspase-3 and cognition of aged mice[J]. Journal of Capital Medical University, 2017, 38(3): 348-351.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.03.004

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I3/348

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