Bio-informative analysis of severe intrauterine adhesion related gene

doi:10.3969/j.issn.1006-7795.2018.05.017

Abstract

Abstract: Objective To detect the mechanism in molecular level of severe intrauterine adhesion(IUA). Methods Thirteen severe IUA tissues and thirteen normal uterus tissues were abtained.Three samples of each group were tested by gene chip.Ten remained samples of each group were used to verified the results of the gene chip by RT-PCR. Then the target genes were subjected to gene ontology and pathway analysis by Go database and Kyoto Encyclopedia of Genes and Genomes (KEGG). Results A total of 1 425 mRNA species were differentially expressed in IUA tissue versus normal uterineus tissue. Of these, 731 mRNAs were highly expressed and 694 mRNAs were lightly minimally expressed in IUA tissues. Further functional analysis showed the function of these gene are involved in signal transduction,multi cell organism development,cell-cell adhesion,cell differentiation,monocyte activation et al.The further analysis indicated the expression level of some mRNAs such as COL16A1, ADAM-9,CDH2, LOX were consistent with the results of gene chip. Conclusion We can use the method of bio-informative analysis to study the data obtained from the gene chip. There are mRNAs that may play a role in the expression and function of extracellular matrix during the formation of IUA.

Key words: intrauterine adhesion, gene chip, bio-informatics

CLC Number:

R711

Liu Xin, Duan Hua, Cheng Jiumei, Chen Fang, Xu Qian. Bio-informative analysis of severe intrauterine adhesion related gene[J]. Journal of Capital Medical University, 2018, 39(5): 715-720.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2018.05.017

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2018/V39/I5/715

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