The expression of NLE1 in colon cancer and its influence on proliferation and apoptosis of HT29 cells

doi:10.3969/j.issn.1006-7795.2021.03.014

Journal of Capital Medical University ›› 2021, Vol. 42 ›› Issue (3): 425-430.doi: 10.3969/j.issn.1006-7795.2021.03.014

• Basic Research • Previous Articles Next Articles

The expression of NLE1 in colon cancer and its influence on proliferation and apoptosis of HT29 cells

Liu Kuiliang^1,2, Li Nanshan^2,3, Wu Jing^1,2*, Li Wenkun², Li Qian², Wang Yadan²

1. Department of Gastroenterology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050;
2. Department of Gastroenterology, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038;
3. Department of Science and Technology, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China

Received:2021-02-08 Online:2021-06-21 Published:2021-06-16
Contact: *E-mail:wujing36@163.com
Supported by:
Outstanding Talents Training Program of Beijing (2017000021469G257), Beijing Municipal Administration of Hospitals’Youth Programme(QML20170704), Beijing Health System Training Program of Senior Talents(2011-RC1).

Abstract

Abstract: Objective s To verify the expression of Notchless homolog 1 (NLE1) in colon adenocarcinoma and evaluate its possible role and mechanism. Methods The expression of NLE1 was evaluated by immunohistochemistry (IHC) staining and quantitative polymerase chain reaction (PCR). Lentivirus transfection was used to explore the influence of NLE1 on proliferation and apoptosis of HT29 and possible mechanism. Results According to IHC, the high expression rate of NLE1 in normal mucosa, colon adenoma and early stage colon cancer was 14.3% (15/105),44.0% (11/25)and 68.6% (72/105),respectively. The expression of NLE1 was significantly higher in cancer compared with adenoma (P<0.05), as well as in adenoma compared with normal mucosa(P<0.001). According to quantitative RT-PCR, NLE1 expression in normal mucosa, colon adenoma and early stage colon cancer was 1.38±0.82,5.04±2.09, 7.57±1.25, respectively. The expression of NLE1 was significantly higher in cancer compared with adenoma (P<0.05), as well as in adenoma compared with normal mucosa(P<0.01). NLE1 silencing significantly inhibited proliferation and colony-forming ability and prmoted apoptosis of HT29 cells(all P<0.05), and increased the expression of Bax and Fas. Conclusions The elevated expression of NLE1 in colon adenoma and adenocarcinoma might promote colorectal carcinogenesis via promoting proliferation of tumor cells.

Key words: NLE1, colon cancer, colon adenoma

CLC Number:

R735.3+4

Liu Kuiliang, Li Nanshan, Wu Jing, Li Wenkun, Li Qian, Wang Yadan. The expression of NLE1 in colon cancer and its influence on proliferation and apoptosis of HT29 cells[J]. Journal of Capital Medical University, 2021, 42(3): 425-430.

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The expression of NLE1 in colon cancer and its influence on proliferation and apoptosis of HT29 cells

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