Changes of the Ultrastructure of Human Hepatocellular Carcinoma Cells and Fibroblasts Cocultured in Vitro

Journal of Capital Medical University ›› 2001, Vol. 22 ›› Issue (1): 10-12.

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Changes of the Ultrastructure of Human Hepatocellular Carcinoma Cells and Fibroblasts Cocultured in Vitro

Zhang Hua¹, Sun Haime¹, Guo Chongjie¹, Gao Fuyun², Zhao Tiande², Wei Yulin²

1. Department of Histology and Embryology, Capital University of Medical Sciences;2. Department of Cell Biology, China Japan Friendship Institute of Clinical Medical Sciences

Received:2000-01-06 Revised:1900-01-01 Online:2001-01-15 Published:2001-01-15

Abstract

Abstract: A localized cell culture method in vitro was empolyed to investigate the interaction between human hepatocellular carcinoma cells and fibroblasts. These two kinds of cells were cocultured in the same container by design.To prepare samples for TEM, embedding slice in situ and vertical sectio ning were adopted. It was convenient to observe the ultrastructure of different kinds of cells with loca lized cell culture. The result indicated that mitochondria appeared to be swollen, the number of lysosomes increased in cytoplasm of the two kinds of cells, and microfilaments beneath the cytomembrane of fibroblasts touching the pseudopodia of the tumor cell were pushed down by pinosomes and broken at some areas after being cocultured for 96 h.The result suggests that the interaction between human hepatocellular carcinoma cells and fibroblasts causes changes of cellular structures.

Key words: human hepatocellular carcinoma cells, fibroblasts, localized cell culture, ultrastructure

CLC Number:

Zhang Hua;Sun Haime;Guo Chongjie;Gao Fuyun;Zhao Tiande;Wei Yulin. Changes of the Ultrastructure of Human Hepatocellular Carcinoma Cells and Fibroblasts Cocultured in Vitro[J]. Journal of Capital Medical University, 2001, 22(1): 10-12.

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Changes of the Ultrastructure of Human Hepatocellular Carcinoma Cells and Fibroblasts Cocultured in Vitro

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