Journal of Capital Medical University ›› 2002, Vol. 23 ›› Issue (4): 307-309.

• 基础研究 • Previous Articles     Next Articles

In Vitro Expression Conditions of Ts87 Antigenic Gene from Trichinella spiralis

Yang Jing, Zhu Xinping, Yang Yaping   

  1. Department of Parasitology, Capital University of Medical Sciences
  • Received:2002-09-04 Revised:1900-01-01 Online:2002-10-15 Published:2002-10-15

Abstract: The influence of the expression conditions, such as the period of inducement, the concentration of inducer and the growth state of the host cell during inducement was investigated in order to increase the level of expression of the fusion protein PET-28a(+)/Ts87/BL21. The results showed that when the inductive conditions were appropriate(OD600 nm =0.6, 37 ℃, 4 h), the amount of the expression product took up over 30% of the total bacterial proteins.

Key words: Trichinella spiralis|Ts87|fusion protein|expression

CLC Number: