Table of Content

15 October 2002, Volume 23 Issue 4

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基础研究

Identification of Differentially Expressed Genes in Hippocampus of Hypoxic Preconditioned Mice

Ma Lufeng;Li Junfa;Li Jingjin;Lü Guowei

2002, 23(4):  289-292. 

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To explore the molecular mechanism of hypoxic preconditioning, isolate and analysis the differential genes from the hippocampus of hypoxic preconditioned mice, the technology of mRNA differential display was used and 56 bands of differential genes were recovered. Fourteen of them were purified, cloned, and sequenced. The BLAST results show that nine of them are known genes, while five of them may be unknown genes. The differentially expressed genes will provide a new clue for further study of the molecular mechanisms of hypoxic preconditioning.

Influence of NGF on Neurons in Dorsal Root Ganglion after an Injure of Peripheral Nerves in Rabbits

Liu Na;Xu Qunyuan;Jing Peng;Cai Qing;Chen Yaliang

2002, 23(4):  293-296. 

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To investigate the effect of NGF on neurons in the dorsal root ganglion(DRG)after a injury of peripheral nerves, a unilateral transaction of the branchial plexus was done in six rabbits with the age of one month. After the transaction, 25 μg NGF was given to the stump end of the nerves before they were sutured and all animals were kept alive for one month. During this period, the rabbits were intramuscularly injected with 12.5 μg NGF every other day. The sizes of the cell bodies and nucleoli and numbers of neurons in the DRG were observed and counted under a light microscope. The ratio of the cell numbers between the injured side and uninjured side were quite close to one in the six rabbits. The nucleoli were taken as a index of calculation of cell numbers. The image analysis was used to measure the sizes of cells and nucleoli in one rabbit. The statistic analysis were used to make comparison of data from different segments and we found that there was no remarkable difference with regard to average diameter of the cells between the injured and uninjured side(P>0.05). There was noremarkable difference on the average diameter of the nucleoli between the two sides either(P>0.05).

Effects of 11, 12-Epoxyeicosatrienoic Acid on the Cardio-Pulmonary Cerebral Resuscitation

Zeng Xiangjun;Zhang Like;Lu Lingqiao;Wang Hongxia

2002, 23(4):  297-299. 

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The objective is to study the effects of 11,12-epoxyeicosatrienoic acid(11,12-EET)on the cardio pulmonary cerebral resuscitation(CPCR). Using the classical method to reproduce the resuscital model, we bring the CPCR into effect at the moment when the heartbeat stopped. Then we observed the succeed rate of resuscitation, the resume rate and time of independent circle and the change of the left heart function. Preconditioning with 11,12-EET or applying 11,12-EET in CPCR can enhance the success rate of CPCR, shorten the resume time of independent circle and improve the heart function after CPCR. The study indicates that 11,12-EET can improve the prognosis of CPCR.

Changes of Electro-mechanical Activity in Duodenum of Cold Restraint Stress Rats

Li Lisheng;Qu Ruiyao;Wang Wei;Guo Hua;Meng Yang

2002, 23(4):  300-302. 

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The aim of this study is to investigate mechanisms of duodenal motility disorders presented in cold restraint stress rats by using electrode and highly sensitive sensor to record alterations of duodenum electromechanical activity. The study suggested that the average frequency and amplitude of duodenal slow wave was lower than the control group(P<0.001); Duodenal motion frequency of cold restraint stress rats was lower than that of the control groups(P<0.001), while motion amplitude was higher than that of the control groups. The time of MMC was obviously less than the control group. The results showed that changes of electro mechanical activity in duodenum of cold restraint stress rat and gastrointestinal peptides may be related to the gastrointestinal motility disorders presented in cold restraint stress rats.

Effect of Hyperbaric Oxygen on Cortical Caspase-3 Activity After Transient Global Cerebral Ischemia In Rats

Tan Yanguo;Chen Rui;Hou Yanzhi;Sun Lin;Yu Peilan

2002, 23(4):  303-306. 

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To investigate the effect of hyperbaric oxygen(HBO)on caspase-3 activity, we devided 200～250 g Sprague-Dawlay rats into three groups: the ischemia/ reperfusion group(I/R), the group treated by HBO and the sham operated group(Sham-O)as the control. Global brain ischemia was induced by using a modified four vessel occlusion model originally described by Pulsinelli and Buchan. Cortex of parietal lobe was obtained and homogenized respectively at reperfusion time points of 6 h, 24 h, 48 h and 96 h after 20 minutes of ischemia. Then caspase-3 activity was measured by cleavage of the colorimetric substrate of DEVD-ρNA. Although both caspase-3 activity of the HBO group and I/R group were not statistically different from the Sham-O group at reperfusion time point of 6 h,the HBO group was higher than the I/R group(P<0.05). Both the HBO group and I/R group were higher than the Sham-O group(P<0.05)at 24 h of reperfusion, but there was no statistical difference between the former two groups. At reperfusion time points of 48 h and 96 h, the HBO and I/R group were higher than Sham-O group, but there was no statistical difference. And there was no statistical difference between the former two groups either, which indicated that caspase-3 activity had decreased to normal levels. The results of above indicated that caspase-3 was activated after global ischemia/reperfusion. HBO treatment at early time may aggravate this activation, but after several times of HBO treatment, this promotion effect becomes indistinct.

In Vitro Expression Conditions of Ts87 Antigenic Gene from Trichinella spiralis

Yang Jing;Zhu Xinping;Yang Yaping

2002, 23(4):  307-309. 

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The influence of the expression conditions, such as the period of inducement, the concentration of inducer and the growth state of the host cell during inducement was investigated in order to increase the level of expression of the fusion protein PET-28a(+)/Ts87/BL21. The results showed that when the inductive conditions were appropriate(OD_{600 nm} =0.6, 37 ℃, 4 h), the amount of the expression product took up over 30% of the total bacterial proteins.

TCSs'Effect on Apoptosis of Human Liver Cancer Cell Line 7721

Zhang Jinfeng;Wang Zhe;Ma Xiaorui;Lin Ziwei;Fu Guifang

2002, 23(4):  310-312. 

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TCSs, one kind of Chinese traditional herbs, is the serials of anticancer medicine. The purpose is to study the mechanism of tumor inhibitory and killing effects of the TCSs on liver cancer cell line 7721. With methods of flow cytometry and Western blotting, we investigated tumor cell apoptosis induced by the herb. It also examined the expression of apoptotic protein with cytochemistry method. The results indicated that the herb can induce tumor cell apoptosis, and the apoptotic rate can reach to 40 percent. After being treated by the herb, the expression of antiapoptosis gene bc1-2 reduced, and proapoptosis gene Bax increased. The results suggest that to induce target cells apoptosis is the main mechanism to inhibit and kill tumor by the herb.

Effect of Pinealectomy on Serum MDA Level in Old Swimming Mice

Zhao Feng;Wang Chuan;Xiong Kaiyu

2002, 23(4):  313-315. 

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The objective was to investigate if melatonin is related with the decrease of MDA by pinealectomy mice during long term aerobic exercise. Pinealectomied 12-month-old mice were divided into three groups: the control, the swimming-60-min and the 60-min with 3% body mass; and normal mice also were divided three groups in the same way. After eight weeks swimming, all animals were killed, and their MDA levels in serum were assayed. Results: In male mice MDA decreased significantly in normal mice than in the pinealectomied in the swimming-60-min and the swimming-60-min with 3% body mass. while in female mice, the swimming-60-min group has shown the same results as above, but in the swimming-60-min with 3% body weight group the level MDA in pinealectomied group decreased significantly than in the normal group. Conclusion: long term suitable intensity aerobic exercise can enhance the activity of antioxidase, and it is related with stimulating pineal and releasing melatonin.

临床研究

Comparision of Culture Methods in Vitro of Rat Embryonic Neural Stem Cells

Wang Hongyun;An Yihua;Wan Hong;Liu Kui;Zhang Yazhuo;Wang Zhongcheng

2002, 23(4):  316-318. 

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Various methods were used to culture rat embryonic neural stem cells(NSCs). The cells were induced to divide so as to be confirmed as NSCs, and were checked by phase contrast microscopy and immunofluorescence. The results showed that cells grew the fastest when both bFGF and EGF were added into the culture medium. Higher NSCs concentration promoted cell proliferation. It suggests that the two neurotrophic factors have cooperative effects on stimulating cell growth and higher NSCs culture concentration can promote its proliferation.

Predictive Survival of Severe Type of Virous Hepatitis: Study on Multiple Risk Factors

Gou Chunyan

2002, 23(4):  319-322. 

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The objective was to analyse multiple risk factors of predictive survive of serious virous hepatitis, and to make a scored system which could determine patient's prognosis. One hundred and seventy patients at the end of hospitalization were divided into the living group(n=45)and the dying group(n=125). Among them 133 patients were men, and 37 patients were women. The average age was 43.5 years old(range from 8 to 79). Twenty clinical and laboratory parameters were investigated as predictive risk factors.Univariate analysis was first made. Variables reaching statistical significance(P<0.05)were subsequently introduced in Logistic regression to identify the independent risk factors for patient's survival. The methods of statistical analysis were performed with SPSS for WINDOWS statistical package. Results: nine clinical and laboratory parameters, PTA, serum Na⁺, hepatic encephalopathy, clinical types of serious virous hepatitis, GI bleeding, syndrome of hepatic kidney, total bilirubin,serum BUN, and ascites were closely related to prognosis of severe type of virous hepatitis by analysis of univariate. Four independent risk factors, PTA, serum Na⁺, hepatic encephalopathy, and clinical types of serious virous hepatitis were obtained by Logistic regression. A scored system was made accroding to the 4 parameters. The threshold score of death was five. The coincidence rate was 80% by retrospective study of 170 patients with serious virous hepatitis. Conclusions: PTA, serum Na⁺, hepatic encephalopathy, and clinical types of serious virous hepatitis are independent predictive factors in patients with serious virous hepatitis. It is important that the risk factors should be assessed, ward off, and treated as early as possible. The treatment strategy can be determined and the medical resources can be reasonably used according to this score system.

Analysis of Polymorphism in Exon 7 of Human Cytochrome P4501A1 Gene in Chinese

Zhang Liqun;Chu Jinhua;Zhou Yan;Zhu Zhangling;Deng Yifu 

2002, 23(4):  323-325. 

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P4501A1 is a kind of enzyme related with the carcinogenic activity of aromatic hydrocarbon, the polymorphism in exons 7 of the gene could alter the function, and has been reported to be associated with lung cancer in Japanese. But data from Caucasian or African-American were different. The difference is said to be from the different frequencies of this polymorphism between ethnic groups. This paper analyzed the frequency of polymorphism in exons 7 of CYP1A1 gene in Chinese by the PCR method. The results suggested that the frequencies of genotype in Chinese were Ile/Ile 61.6%, Ile/Val 26.4% and Val/Val 12.0%, no significant difference of the frequencies between male and female was found. But the frequency of Val/Val was much higher than that in Japanese, Caucasian or African American.

Clinical Evaluation of EF and FS as a Predictor of Preoperative Ventricular Arrhythmia

Gu Song;Su Pixiong;Liu Yan;Yan Jun;Zhang Xitao;Geng Bo;Hu Dayi

2002, 23(4):  326-329. 

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In order to evaluate the accuracy of left ventricular ejection fraction(EF)and fractional shortening(FS)for predicting malignant ventricular arrhythmia(VA)after coronary artery bypass graft(CABG). One hundred and fifty consecutive patients were selected for an isolated CABG procedure, EF and FS, left ventricular potential(LVP), myocardial enzymes and 24 h Holter were measured in special perioperative period. 1)Patients with previous myocardial infarction(MI), aneurysm, positive LVP had obviously lower value of EF and FS before CABG than those without them. 2)In patients with LVD, significant improvements were found in EF and FS indexes after CABG. 3)The incidence of postoperative VA is higher in these patients with LVD, VA, VLP+ and aneurysm than the others. The results showed that: 1)EF and FS were sensitive indexes in reflecting left ventricular function. 2)CABG and aneurysmectomy not only improved myocardial blood supply, but also increased the heart out put, and brought the best benefits to those patients with LVD. 3)For the reason of myocardial depression, the cardiac function of patients without LVD decreased in short term follow up(14±1)d. 4)EF≤40% and/or EF≤24% were independent predictors for the prevision of malignant VA, the value of FS accurately reflected left ventricular function. 5)Integrate with other indexes, such as LVD, VLP and aneurysm, would improve the sensitivity, specialty and accuracy for predicting malignant VA after CABG.

Study of Renal Transplantation in Aged Uremic Patients

Wang Jianwen;Gao Juzhong;Han Xiuwu;Han Zhiyou;Li Xiaobei;Zhang Yong;Liu Jing;Guan Delin

2002, 23(4):  330-332. 

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Fifty one uremic patients over 60 who received cadaveric renal transplantation were retrospectively analyzed in order to investigate the renal transplantation in the aged uremic patients. Results: the survival rate of person/kidney one year was 88.2%/86.2%. Two patients died from the acute tubular necrosis and heart failure and lung infection, one patient died from the severe pneumonia while the other one died from cerebrovascular accident. The total complications post transplantation included one hyperacute rejection, four acute rejection, two chronic rejection, four acute tubular necrosis, 13 hepatotoxicity and nephrotoxicity of cyclosporin A, six leukopenia, 2 posttransplant diabetes mellitus, 9 lung infection, eight heart failure and one fistula in the ureterocystanastomosis. Therefore, the advanced patients are still suitable for the renal transplantation. It special attention has to be paid on the treatment of infection and cardiovascular diseases and nursing care, and it is also important to adjust and monitor the toxic side effects of the immunosuppressive drugs, and the dose of CsA in the aged patients should be lower.

Lateral Locatin Diagnosis Value of PET in Temporal Lobe Epilepsy

Sun Zhengrong;Luan Guoming;Zhang Bin

2002, 23(4):  333-325. 

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To study the lateral location diagnosis value of PET in temporal lobe epilepsy before operation, 20 patients with refractory temporal lobe epilepsy were investigated by EEG, MRI and PET. Nineteen of 20 patients had epileptiform discharges in EEG scalp recordings, but only one patient had normal EEG. EEG recordings showed unilateral EEG abnormal focus in 10 patients and bilateral abnormal focus in nine patients. MRI demonstrated 12 normal, five unilateral hippocampal atrophy, one left parietal occipital softening and one left frontal heterotopic gray matter and one rignt frontoparietal cyst accompanied with pituitary adenoma. PET revealed focal hypometabolism in all 20 patients, of whom 16 had unilateral abnormalities and 4 bilateral abnormalities. All patients were treated by operation, 16 patients underwent anterior temporal resection and hippocampectomy, 4 patients selective hippocampectomy. The pathologic examination showed gliosis and neuron death in resected samples. These results indicates that PET is superior to MRI in localizing diagnosis of temporal lobe epilepsy, more sensitive and reliable.

Clinical and Electrophysiological Study of Myotonic Dystrophy

Zhao Xiaoling;Wang Xianling;Xing Huafang

2002, 23(4):  336-338. 

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To study clinical and electrophysiological features in myotonic dystrophy. Methods: Electromyography(EMG)and nerve conduction velocity were performed in twenty-four patients with myotonic dystrophy. Results: In 46% of cases,(motor/or sensory)nerve conduction velocity was abnormal in at least two separate nerves. It was characterized by mildly slowing motor/or sensory nerve conduction and reducing amplitude of compound motor action potential(CMAP)/or sensory nerve action potential(SNAP). The severity of disease was associated with the patient's age. Conclusion: Peripheral neuropathy in myotonic dystrophy is not rare. It is a sensorimotor axonal peripheral neuropathy. The clinical symptoms are worse in older patients.

Preoperative Evaluation of Hip Fractures in Aged Patients

Shi Yongchang;Yu Qiang

2002, 23(4):  339-341. 

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To discuss the estimate ways of preoperative aged patiens′with hip fracture, from 1988 to 1998, 68 patients over 75 with hip fracture were examired before operation. Their whole state induding the function of heart, lung, liver, kidney and blood pressure, anaemia, blood sugar, whole protein, eletrolyte and CO₂CP and discuss the feasibility indication、anaesthesia、perioperative period were evaluated by scores. The grades of patients′whole body states were from 55 to 100, average 76.3. The operation team was from 70 to 100, average 82.8. The nonoperation team was from 55 to 75, average 66.5. The difference was statistically significant between the age team over 85 and other age teams(P<0.01). The effective rate of operation team was 75.7%. The grade of routine examination results have clinical worth for the estimation of whole state of the old patients and prognosis.

Study on Different Dosages of Misoprostol in Painless Induced Abortion

He Xinhong;Lin Li;Hu Yuquan

2002, 23(4):  342-343. 

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Induced abortion with propofol anaesthesia avoids patients from pain, it is a safe and effectual method to terminate pregnancy. However, propofol can not make cervix uteri relax, so the difficulties and complications from cervix uteri in induced abortion can not be resolved. In this study, the dosages and time of taking misoprostol orally before operation was studied further. It was proved that cervix uteri in experiment groups was much flabbier than that in control group, if women took misoprostol 200 μg or 400 μg orally 2 h before the operation. The effect was obvious. The method is worth popularizing.

Comparison Between Creatinine's Enzymatic Method and Kinetic Jaffe's Method and Interference Factors Analysis

Jin Ye;Su Jianrong

2002, 23(4):  344-345. 

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The objective was to compare and investigate the interference and the difference of Creatinine's enzymatic method and kinetic Jaffe method. To measure creatinine of serum or plasma, Beckman CX 7 analyzer with Beckman reagent for kinetic Jaffe method and Randox reagent for enzymatic method were used. The interferences of lipemia, hemoglobin and bilirubin were compared. The results showed that enzymatic method was not interfered, Jaffe method was negatively affected by bilirubin(>158 μmol/L). Creatinine's enzymatic method is better than kinetic Jaffe method.