Table of Content

15 January 2003, Volume 24 Issue 1

Previous Issue    Next Issue

论著·基础研究

Dynamic Changes of Cell Proliferative Rates and Expression of Basic Fibroblasts Growth Factor in Serum-free Culture of Human Hepatocellular Carcinoma in Vitro

Zhang Hua;Guo Chongjie;Jing Peng;Gao Fuyun;Zhao Tiande

2003, 24(1):  1-3. 

Asbtract ( 623 )   PDF (208KB) ( 277 )  

Related Articles | Metrics

The objective was to study the mechanism of short range modulation of carcinoma cells in serum free culture medium. The cell measurement in situ and immunohistochemical staining were employed to observe the dynamic changes of cell proliferative rate and the expression of basic fibroblasts growth factor (bFGF) of human hepatocellular cells that were cultured in serum-free and mitogen-free medium. Results: With the changes of cell proliferative rate, the dynamic changes of expression of bFGF at different phases were found. When the proliferative rate was increased, the expressions of bFGF increased strongly, and vice versa. These suggest that the autocrine and paracrine growth factors play an important role in short range modulation of cell proliferation.

Effects of Mn²⁺ on Rat Liver Nuclei

Huang Peili;Zhang Shuhua;Zhu Jinhua;Li Guojun;Li Jianxin

2003, 24(1):  4-6. 

Asbtract ( 685 )   PDF (160KB) ( 213 )  

Related Articles | Metrics

To study the effects of Mn²⁺ on the rat liver nuclei pre treated with Mn²⁺ , AAS, MFS and UV were used to determinate the changes of contents of Mn, malondialdehyde(MDA) and superoxide dismutase(SOD) in rats. The results obtained by spectrophotometic method, showed that the contents of Mn,MDA and SOD injected Mn were higher significantly than in untreated animals ( P< 0.01 ). Mn can be uptaken by rat liver cells, and in the nuclei there are many effects on the lipid peroxide and systems of antioxide.

Production and Characterization of an Antiserum Against Human α-synuclein

Liu Xuemei;Yu Shun;Li Yaohua;Zhou Ming;Zuo Xiaohong;Lü Guowei;Cheng Biao

2003, 24(1):  7-10. 

Asbtract ( 529 )   PDF (232KB) ( 210 )  

Related Articles | Metrics

To develop an antiserum specific to human α-synuclein for the study of α-synuclein related neurodegenerative diseases and pathological conditions, BALB/C mice were immunized with recombinant human α-synuclein. The antiserum was characterized by Dot blot, Western blot and immunohistochemistry. Dot blot showed that the antiserum could detect human recombinant α-synuclein at concentration 52ng. Western blot analysis revealed that the antiserum could recognize a 18000 protein from human and rat brain homogenates, which was consistent with the reported molecular weight of the protein. Immunohistochemical staining with the antiserum showed that α-synuclein immunoreactivity was mainly distributed in cortex, hippocampus, striatum, amygdala and olfactory bulb, and was concentrated in the nerve terminals and cytoplasms near the nuclear envelopes of neurons. The antiserum can specifically recognize the α-synucleins in human and rat brains and can be used in α-synuclein related diseases.

Streptococcal Histone-Like Protein Induces Murine Peritoneal MΦ to Produce IL-6

Shen Haizhong;Zhang Liping;Ping Guoling;Zhang Hongchun;Jing Xuefang;Li Yuying;Li Yulan;Li Weihong;Zhang Haiyan

2003, 24(1):  11-13. 

Asbtract ( 640 )   PDF (162KB) ( 224 )  

Related Articles | Metrics

To evaluate the effect of Streptococcus HlpA on interlukin-6(IL-6)production by murine peritoneal MΦ, HlpA and/or LTA were added to the cultured MΦ and maintained for different time periods. IL-6 activity was measured by means of 7TD1 cells. The results showed that the IL-6 production from MΦ induced by HlpA of Streptococcus was time and concentration dependent in vitro. There was a synergisticly increased response on the production of IL-6 induced by the mixture of HlpA and LTA, as compared to HlpA alone. On the contrary, the addition of heparin could deduce the IL-6 production.

Treatment of Limb Ischemia in Rabbits by VEGF Gene

Teng Xu;Qi Yahui;Wu Wenqi;Bing Guoying;Cui Shijun;Yu Hengxi

2003, 24(1):  14-18. 

Asbtract ( 732 )   PDF (240KB) ( 232 )  

Related Articles | Metrics

The objective was to study the gene therapy effects of vascular endothelial growth factor(VEGF) and to find the new approaches to describe the development and evaluation of blood circulation on a rabbit hindlimb ischemic model. The limb ischemic model was developed by ligation of the distal external iliac artery and excision of every branches of femoral arteries. As soon as the model was established, the recombinant plasmids pcDNA3.1/VEGF165 was transferred by injection into the ischemia muscles. Each animal was evaluated by angiography, calf blood pressure, blood volume of keen per second, speed of blood circulation and erythrocyte concentration per second on keens of the rabbit. At the same time the histologic changes of the adductor muscle and the gastrocnemucs muscle in the left and right hindlimbs were studied. In control group the number of the rabbits which developed superficial tissue necrosis in the foot was greater than those in experimental group. Angiography demonstrated that in the experimental group, the filling of the distal arterial was markedly faster than in the control group and focal neovessels establishment of collateral circulation was higher than that in the control group at the same time. The calf blood presure in the experimental group increased faster than that in the control group. Histologic study showed that the evidences of atrophy and fibrosis in the control group were more obvious than those in the experimental group and neovessels were less than the experimental group. Between the two groups there were not significant difference in the function of liver and kidney. The experimental studies indicate that VEGF gene therapy would be effective and safe for limb ischemia diseases.

Effect of Chinese Herb on NOS Expression in Dorsal Root Ganglia in Rats with Spinal Cord Hemisection

Ma Yuping;Gao Xiulai;Chen Yaliang;Lu Tao;Song Yizhi;Jing Peng;Han Fengyue;Su Jing;Liu Weidong;Zhang Ping;Chang Chaoying;Liu En

2003, 24(1):  19-22. 

Asbtract ( 865 )   PDF (462KB) ( 292 )  

Related Articles | Metrics

The object was to study the effect of Chinese Herb I(SCI) on NOSexpression in dorsal root ganglia (DRG) in rats with spinal cord hemisection and to explore the mechanism of spinal cord regeneration promoted by SCI. NADPH-d histochemical method and image analyses were used to detect changes of NOS, which was evoked by spinal injury in Wistar rats. The result indicated that evoking NOS was strengthened by the administration of SCI as compared with that of the control group. The up regulation of NOS was heightened continuously in DRG of the injury side and the strong positive findings nearly exceeded 40%. This study shows that the magnitude of NOSis associated with spinal regeneration and repair, and SCI can regulate NOS expression and promote regeneration of the spinal cord.

Expression of Lumbrukinase PI₂₃₉ Gene in the Insect Cell using Baculovirus Expression Vector System

Shen Yue;Liang Hong;Sun Zhaojun;Fu Shihon;Liang Guodong

2003, 24(1):  23-26. 

Asbtract ( 624 )   PDF (400KB) ( 304 )  

Related Articles | Metrics

The objective of the study was to better understand the thrombolytic enzyme in earthworm (lumbrukinase). By using the baculovirus expression vector system, PI₂₃₉ protein was expressed in the insect cell. The PI₂₃₉ gene was inserted into the donor plasmid pFastbac. Through transposition, recombinant bacmid PI₂₃₉ DNA was formed and was then transfected into Sf9 insect cells to produce recombinant Baculovirus. Sf9 insect cell were infected with recombinant Baclovirous. The harvested culture supernatant and cells lysate occured after 72h, the SDS-PAGE and Western Blot analysis. The SDS-PAGE analysis revealed that molecular weight of expressed protein was 30000. Western blot showed that PI₂₃₉ protein could be recognized by specific antiPI₂₃₉ antibody. The PI₂₃₉ protein can be expressed on the insect cell Sf9.

Effects of Flaxseed on the Content of DHA in Egg Yolk and Tissue

Wang Hui;Zhang Shuhua;Yin Na;Guo Aimin;Yan Fusheng

2003, 24(1):  27-29. 

Asbtract ( 553 )   PDF (374KB) ( 344 )  

Related Articles | Metrics

The objective was to study the effects of flaxseed on the content of DHA in egg yolk and tissue. The laying hens were fed on diet containing flaxseed for 10 weeks. Analysis of gas chromatography showed that, as compared with control eggs, DHA content of yolk in the dose group increased evidently. The study also found that DHA content of liver and brain in the dose group increased accordingly. These results indicate that flaxseed is valuable for exploiting new hygienical food.

Application of Genetic Algorithm in Medical Image Registration

Shen Jinhui

2003, 24(1):  30-32. 

Asbtract ( 556 )   PDF (304KB) ( 345 )  

Related Articles | Metrics

An genetic algorithm based image affine registration method was designed. The possiblilty of using different code methods at different stages was discussed.Float-code can help genetic algorithm to escape from local entrapment and gray code can get the final value quickly.The result proves that this is an efficient and superior method.

Protection of Liquid Extract of Jiunaoyizhi Capsule Against Impairment of Hippocampal Neurons of D-galactose-Induced Aging Mice

Zhao Zhiwei;Wang Pengwen;Song Lina;Zhuang Xiaoming;Wang Yuanshen;Ji Zhijuan;Sheng Shuli

2003, 24(1):  33-36. 

Asbtract ( 609 )   PDF (229KB) ( 229 )  

Related Articles | Metrics

The objective of this study was to investigate whether the liquid extract of Jiunaoyizhi capsule has a protective effect on neuronal impairment of the aging mice induced by D-galactose. Several antibodies were applied to detect the immunoreactive positive neurons in hippocampus. It was found that in the D-galactose induced aging mice the Akt/PKB, CREB and Bcl-2 is as well as PP-1 positive neurons decreased obviously, but AIF and Bax increased significantly. The mice treated with 0.3 g/kg liquid extract of raw herbs could make these changes normal. The small dosage of jiunaoyizhi has effect on the impairment of signal pathway of hippocampal neurons in the aging mice. But the unexpected result was that the 1.0 g/kg liquid extract exerted no such effect.

Changes of Platelet Membrane Glycoprotein in Mice after Cerebral Ischemia-Reperfusion

Wang Guozhong;Gao Chunjin;Ge Huan;Wang Shaoting;Xia Chengqing;Qi Man;Chen Rui

2003, 24(1):  37-39. 

Asbtract ( 688 )   PDF (162KB) ( 184 )  

Related Articles | Metrics

Cerebral ischemia-reperfusion models were established by clamping both common carotid arteries in mice. With the flow cytometry, the expressions of CD₃₁,CD₆₁ and CD_62p on platelet membrane in model mice were measured at the 3rd h, 6th h, 12th h, 24th h, 48th h, 96th h after reperfusion. The expression of CD₃₁ on platelet membrane in mice increased at the 3rd h after reperfusion, reached the peak at the 24th h, and then decreased gradually, but the upregulation of CD₃₁ expression still existed at the 96th h. The expression of CD₆₁ on platelet membrane in mice increased at the 3rd h after reperfusion, lasted to the 24th h, and then returned to normal level at the 48th h. The expression of CD_62p on platelet membrane in mice increased at the 3rd h after reperfusion, reached the peak at the 12th h, and then decreased gradually, but maintained a higher level at the 96th h. The results indicate cerebral ischemia reperfusion can lead to the upregulation of CD₃₁ , CD₆₁ and CD_62p expression on platelet membrane in ischemia reperfusion mice, then may activate the platelets, and result in thrombosis.

Effect of APP-17 Peptide on Modulating Neuronal Mitochondria Transmembrane Potentials in Hippocampus of STZ-DM Rats

Li Hongxing;Wang Rong;Du Yifeng;Ji Zhijuan;Sheng Shuli

2003, 24(1):  40-43. 

Asbtract ( 611 )   PDF (200KB) ( 256 )  

Related Articles | Metrics

The objectives were to investigate the role of the alteration of mitochondria transmembrane potentials in apoptosis in hippocampal neurons of STZ-DM rats and the possible effect of APP17 peptide on it. DM models were established by injection of streptozotocin intraperitoneal, the APP17 peptide treated group were injected with APP17 peptide three times a week, whereas the control and DM groups were given saline. Single cell of hippocampus was isolated, and mitochondria transmembrane potentials and the percentage of cell in apoptosis were measured by means of flow cytometry (FCM) at the 11th week when the animals were sacrificed. The neurons in hippocampus of DM group had lower mitochondria transmembrane potentials and higher apoptotic rate than those of the control group. APP-17 peptide could significantly blocked the mitochondria transmembrane potentials collapse and apoptosis. Decreased mitochondria transmembrane potential and related apoptosis may contribute to the mechanism of diabetic encephalopathy. APP-17 peptide palys an anti apoptosis role in it.

论著·临床研究

Analysis of 5-year follow-up on Microalbuminuria in Patients with Disorder of Glucose Metabolism

Li Hongbing;Wu Baoyu;Yuan Shenyuan

2003, 24(1):  44-46. 

Asbtract ( 725 )   PDF (150KB) ( 154 )  

Related Articles | Metrics

To explore the relative importance of risk factors on microalbuminuria in the patients with disorder of glucose metabolism, a 5-year follow up of 541 patients with disorder of glucose metabolism, aged (56±11) years at baseline with UAER<20μg/min was carried out. The cases of UAER ≥20μg/min were 20.9% (113/541). Key risk factors included hypertensive duration, blood pressure, or smoking. Independent risk factors, as assessed by multiple stepwise regression effects, were SBP(P=0.000), OGTT 2h blood glucose (P=0.014). Microalbuminuria is significantly correlated with blood pressure and hyperglycemia, which suggests that early intervention is necessary.

Relationship between Glycosylated Low Density Lipoprotein and Coronary Artery Disease

Wang Qing;Hu Dayi;Wang Jieping;Yin Zhaoxia

2003, 24(1):  47-50. 

Asbtract ( 736 )   PDF (186KB) ( 210 )  

Related Articles | Metrics

The objective of the study was to observe the changes of glycosylated low density lipoprotein (Gly-LDL) in patients with coronary artery disease (CAD) and in patients with CADcomplicated type-2 diabetes mellitus. The levels of Gly-LDL index were measured by nitroblue tetrazolium reduction technique in sixty eight patients with CAD. Among patients with CAD, there were twenty eight patients with type 2 diabetes mellitus and forty patients without diabetes mellitus. The levels of Gly-LDL index were significantly higher in patients with CAD than those without CAD (P< 0.01). Among the CAD group, the levels of Gly-LDL index were higher in patients with type-2 diabetes mellitus than those in patients without diabetes mellitus (P<0.05). The result indicates the levels of Gly-LDL index increase in patients with CAD and they have a significant increase in patients with CAD complicated type-2 diabetics. Gly-LDL may be one cause of the accelerated atherogenesis in type-2 diabetic patients and may be indirect biochemical marker of the development of macroangiopathy in type-2 diabetic patient.

Protection Effect of Ischemic Preconditioning is Less Obvious in Elderly Patients

Wan Xinhong;Liu Xiaohui;Yang Xinchun

2003, 24(1):  51-53. 

Asbtract ( 649 )   PDF (152KB) ( 173 )  

Related Articles | Metrics

The objectives of the study were to examine whether angina 48 h before acute myocardial infarction provided protection in elderly patients and to retrospectively verify whether preinfarction angina within₄₈ h of the first myocardial infarction exerted a beneficial effet on in hospital outcomes in adults(≤45 years old, n=104)and elderly(≥65 years old and ≤75 years old, n=134)patients. In hospital congestive heart failure (8 vs 1, 14.8 % vs 2.0%, P=0.048), cardiogenic shock(8 vs 1, 14.8 % vs 2.0%, P =0.048) and left ventricular dysfunction (11 vs 2, 20.4% vs 4.0%, P=0.012)were more frequent in adult patients without than in those with previous angina. In contrast, the presence or absence of preinfarction angina did not seem to influence in hospital outcomes in elderly patients. The protective effect from preinfarction angina seemed to be lower in elderly patients than in adults. This study suggests that the absence of preconditioning probably results in the poor in hospital outcomes in elderly patients.

Relationships between Retinal Arteriosclerosis and Essential Hypertension

Di Rujie;Hua Qi;Xing Xiourong;Pi Lin;Wu Hang

2003, 24(1):  54-55. 

Asbtract ( 533 )   PDF (186KB) ( 207 )  

Related Articles | Metrics

The aim of the present study was to assess the risk of retinal arteriosclerosis at different ambulatory blood pressures (ABP) in essential hypertensive patients. Subjects were males and females, aged between 14 and 73 years, suffering from mild to moderate essential hypertension. BPdata was obtained by using Spacelabs monitors for a full 24 h. The retinal artery were examined using fundscope. The results showed significant differences in the degree of retinal arteriosclerosis between groups with different clinical diastolic blood pressure (DBP) and different DBPL (P<0.05). No similar finding was between retinal artery and the difference of pulse pressure and night BPdecreasing rate. The proportion of first grade retinal arteriosclerosis in hypertensives patient was 62.4%. It is suggested that retinal arteriosclerosis(gradeⅠ) was more closely related with DBP in most of the subjects suffering from mild to moderate essential hypertension, coexisted with LVH.

Age Influnces on Recovery Outcome of Spinal Cord Injury Treated by Intraspinal Transplantation of OECs

Huang Hongyun;Chen Lin;Wang Hongmei;Xiu Bo;Li Bingchen;Wang Rui;Zhang Jian;Zhang Feng;Gu Zheng;Li Ying;Song Yinglun;Hao Wei

2003, 24(1):  56-59. 

Asbtract ( 686 )   PDF (196KB) ( 238 )  

Related Articles | Metrics

Objective: To compare the recovery outcome of patients among different ages with spinal cord injuries(SCI) treated by intraspinal transplantation of olfactory ensheathing cells(OECs). Methods: Olfactory bulbs were obtained and trypsinized to be single OECs; which were cultured for about 2 weeks, then transplanted into the upper and lower sites between the injury and the normal spinal cord. 171 patients with the spinal cord injury were treated from half a year to eighteen years after trauma. There were 139 males and 32 females with ages ranging from 2.5 to 64 years (mean 34.9 years). They were divided into 5 groups, 9 patients in the group of less than 20 years old age, 54 in the group of 21 to 30 years, 60 in the group of 31 to 40 years, 34 in the group of 41 to 50 years and 14 in the group of more than 51 years. The spinal cord function was assessed by using the American Spinal Injury Association (ASIA) Classification System before and 2 to 8 weeks after operation. Statistical significance levels were calculated using a one way ANOVA paired Ttest. Results: The motor score of ASIA motor and sensory scale increased by 5.2±4.8, 8.6± 8.0, 8.3±8.8, 5.7±7.3 and 8.2±7.6 in the 5 groups after operation respectively. Light touch score increased by 13.9±8.1, 15.5±14.3, 12.0±14.4, 14.1 ±18.5 and 24.8±25.3 respectively. Pin prick score increased by 11.1±7.9, 17.2±14.3, 13.2±11.8, 13.6±13.9 and 25.4±24.3 respectively. Pin prick in over 51 years groups better than other groups(P< 0.05) except 21～30 years group. Conclusion: OECs transplantation can improve the neurological function of the spinal cord of all patients following SCI regardless of age. The age of injury does not affect ASIA Score for motor and light touch except better recovery for pin prick in over 51 years group. However, further research into the long term outcome of the treatment will be required.

Skull base Chordoma: Ultrastructure and Clinical Research

Sun Yilin;Zhang Hui;Qu Baoqing;Wang Zhongcheng;Zhang Junting

2003, 24(1):  60-63. 

Asbtract ( 581 )   PDF (258KB) ( 243 )  

Related Articles | Metrics

Fourty-one patients with intracranial chordoma near the base of the skull (including 2 cases chondroid chordoma) are reported along with the clinical features and histopathology and ultrastructure character. This tumor had morphologic features identical to a chordoma: nodular growth with vacuolated cytoplasm and myxomatous stroma by light microscopy, positive immunoreaction for cytokeratin and epithelial membrane antigen by immunohistochemistry, desmosomes, microvilli mitochondria surround by rough endoplasmic reticulum by electron microscopy. The fine structure feature of chondroid chordoma was microtubules which located within the rough endoplasmic reticulum. The ultrastructural research confirmed that the chordoma presented not only epithelial differentiation but also mesenchymal. Some cells were multipotential neoplasms cells in which the stem cells were capable of differentiation along both epithelial and mesenchymal pathways. The results suggest the possibility of altered differentiation pathway of the tumor stem cell emergence of a new malignant cell population within the chordoma.

Effect of Endogenous Nitric Oxide on Lung Injury Associated with Acute Pancreatitis in Rats and its Relationship to Oxidative Stress

Zhang Shuwen;Zhang Zaixing;Li Fei;Sun Jiabang

2003, 24(1):  64-66. 

Asbtract ( 651 )   PDF (160KB) ( 201 )  

Related Articles | Metrics

To investigate the effect of endogenous nitric oxide (NO) on lung injury in acute pancreatitis in rats and its relationship to sulfhydryl compounds and lipid peroxidation. Methods: Acute pancreatitis lung injury model in rats was induced by retrograde sodium taurocholate (5%) infusion into the pancreatobiliary duct (1 mL/kg), and NGnitro Larginine (L-NNA) was used as the inhibitor of endogenous NO. The effect of endogenous NO on lung injury, lung tissue levels of sulfhydryl compounds and malonaldehyde (MDA, the end product of lipid peroxidation), were evaluated respectively. Results: Sodium taurocholate administration produced evident lung injury associated with acute necrosis pancreatitis in rats, a decrease of sulfhydryl compounds and a increase of MDA in lung tissue. Pretreatment with the NO inhibitor, L-NNA(12.5 mg/kg), significantly intensified lung injury, and resulted in a further increase of lung tissue MDA, but it had no effect on the lung tissue sulfhydryl compounds. Conclusion: Endogenous NO has the effect of lung protection, and its antioxidation may be responsible, at least in part, for the protective mechanisms. Sulfhydryl compound may not be involved in NO lung protection mechanisms.

Hemodynamic Effects of Neostigmine and Atropine used for Reversing Muscular Relaxation

Li Yan;Zhou Xiaoli;Wang Baoguo

2003, 24(1):  67-70. 

Asbtract ( 927 )   PDF (301KB) ( 278 )  

Related Articles | Metrics

The objective was to observe the hemodynamic changes after intravenous injection of neostigmine and atropine used for reversing the muscular relaxation. Methods: Twenty one neurosurgical patients, ASA class Ⅰ～Ⅱ, were randomly allocated into two groups. The mixture of neostigmine and atropine with 2∶1 (0.05 mg/kg∶0.025 mg/kg) in group Ⅰ or 3∶1 (0.05 mg/kg∶0.017 mg/kg) in group Ⅱ was given intravenously at the end of surgery with isoflurane pipecuronium anesthesia. The hemodynamic parameters were measured with transesophageal Doppler monitor (HEMOSONICTM 100) and Datex Cardiocap. Result: In both groups, HR increased significantly at 1, 2, 3 min after injecting the mixture of neostigmine and atropine. HR and peak velocity (PV) decreased significantly at 30 min after injection in group II. Other hemodynamic parameters did not change significantly after neostigmine and atropine were administered intravenously. Conclusion: 0.05 mg/kg neostigmine and 0.025 mg/kg atropine appear to be a better combination used for the neuromuscular reversal.

Diagnosis and Treatment of Female Bladderneck Obstruction

Su Yong;Zhou Yongjian;Han Zhixing

2003, 24(1):  71-72. 

Asbtract ( 632 )   PDF (116KB) ( 222 )  

Related Articles | Metrics

To study the methods of diagnosis and treatment of female bladder neck obstruction, 24 cases of female bladder neck obstruction were reviewed. All cases underwent transurethral resection of the semicircle of bladder neck. No incontinence or fistula has been noted. Cystourethroscopy with urodynamic study is a reliable diagnostic method and transurethral resection of semicircle of bladder neck is a better therapeutic option.

Treatment of Spastic Cerebral Palsy by Local Injection with Botulinum-A Toxin

Gao Baoqin;Yang Weili;Zhu Bangjie;Deng Xin;Wang Yajie;Deng Yaxian;Tian Zhaoxia;Wang Guifen

2003, 24(1):  73-75. 

Asbtract ( 578 )   PDF (330KB) ( 219 )  

Related Articles | Metrics

To evaluate the efficacy of local intramuscular injections of three different doses of botulinum Atoxin (B-TXA) in the management of the spasm of cerebral palsy. Method: 120 cases of children with cerebral palsy were divided into three average groups: group 1, group 2 and group 3. Three different doses of BTX-A (group 1, 1 U/kg; group 2, 2 U/kg; group 3, 3 U/kg) were administered. The spastic muscles were identified by palpitation. The effect of treatment was evaluated by PRS method. The different efficacy between each group was calculated by analysis of variance. The tolerance to BTX-A and the side effects were also assessed. Result: Reduction on spasm of muscles in all patients (100%) became apparent after injection (P<0.01). There is significant differences when the three different doses group was compared one by one (see table). Larger quantity of BTX-A(2 U/kg, 3 U/kg) is more effective than that of less quantities. No major side effects occurred. Larger quantity of BTX Atreating cerebral palsy is more effective than that of smaller quantity. It is safe when the dosages are in a suitable range (≤6 U/kg).