Expression of Lumbrukinase PI239 Gene in the Insect Cell using Baculovirus Expression Vector System

Journal of Capital Medical University ›› 2003, Vol. 24 ›› Issue (1): 23-26.

• 论著·基础研究 • Previous Articles Next Articles

Expression of Lumbrukinase PI₂₃₉ Gene in the Insect Cell using Baculovirus Expression Vector System

Shen Yue¹, Liang Hong¹, Sun Zhaojun², Fu Shihon², Liang Guodong²

1. Clinic Science and Technology Center, Capital University of Medical Sciences;2. National Point Laboratory of Genetic Engineering, Virus Graduate School, Prevention in China Medical Science Section College

Received:2002-01-05 Revised:1900-01-01 Online:2003-01-15 Published:2003-01-15

Abstract

Abstract: The objective of the study was to better understand the thrombolytic enzyme in earthworm (lumbrukinase). By using the baculovirus expression vector system, PI₂₃₉ protein was expressed in the insect cell. The PI₂₃₉ gene was inserted into the donor plasmid pFastbac. Through transposition, recombinant bacmid PI₂₃₉ DNA was formed and was then transfected into Sf9 insect cells to produce recombinant Baculovirus. Sf9 insect cell were infected with recombinant Baclovirous. The harvested culture supernatant and cells lysate occured after 72h, the SDS-PAGE and Western Blot analysis. The SDS-PAGE analysis revealed that molecular weight of expressed protein was 30000. Western blot showed that PI₂₃₉ protein could be recognized by specific antiPI₂₃₉ antibody. The PI₂₃₉ protein can be expressed on the insect cell Sf9.

Key words: lumbrukinase, baculovirus, express

CLC Number:

R392.1

Shen Yue;Liang Hong;Sun Zhaojun;Fu Shihon;Liang Guodong. Expression of Lumbrukinase PI₂₃₉ Gene in the Insect Cell using Baculovirus Expression Vector System[J]. Journal of Capital Medical University, 2003, 24(1): 23-26.

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Expression of Lumbrukinase PI₂₃₉ Gene in the Insect Cell using Baculovirus Expression Vector System

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