Abstract: Objective To establish the model of primary cultured mice embryonic cardiomyocytes,which is adapted to isolation,culture and identification. Methods To isolate hearts from different day gestational age mice,then digested,seeded,proliferation in vitro and identified in different ways. Results Repeat digestion with 0.125% trypsin and 0.02% EDTA,then in the form of cell suspension seeded in tissue culture plate precoated with fetal bovine serum,and added with Ham F12 containing 20% FBS(pH 7.2⁷.4),and finally identified cells with PAS staining and immunocytochemical methods,which proved the rate between cardiomyocytes and cultured cells was about 80%.Conclusion The model of cultured mice embryonic cardiomyocytes has highly effective.

Key words: myocardial cells, primary cultured