Inhibition of TRIM29 gene by siRNA suppresses the proliferation and migration of NCI-H520 lung cancer cells

doi:10.3969/j.issn.1006-7795.2014.06.008

Abstract

Abstract:

Objective To study the effects of TRIM29 gene on the proliferation and migration of human lung cancer cell line NCI-H520. Methods We transfected TRIM29 siRNA into NCI-H520 cells. Real time reverse transcriptase polymerase chain reaction and Western blotting assay were employed to determine TRIM29 messenger (m)RNA and protein expressions. MTT assay was used to investigate the proliferation of the cells, and the cell migration was evaluated using a transwell migration assay. Results The mRNA and protein levels of TRIM29 were significantly downregulated at 48 h after transfection in TRIM29 siRNA group compared with the control groups. The cell proliferation and migration were significantly reduced. Conclusion siRNA targeting TRIM29 gene can inhibit the proliferation and migration of human lung cancer cell line NCI-H520.

Key words: lung cancer, RNA interference, TRIM29 protein, cell proliferation, cell migration

CLC Number:

R734.2

Liu Chunxiao, LI Hui, Hou Shengcai, Hu Bin, Miao Jinbai, Zhang Wenqian. Inhibition of TRIM29 gene by siRNA suppresses the proliferation and migration of NCI-H520 lung cancer cells[J]. Journal of Capital Medical University, 2014, 35(6): 717-720.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2014.06.008

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2014/V35/I6/717

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