Molecular Cloning of cDNA of Dopamine D2 Receptor from Human Fetal Brain

Journal of Capital Medical University ›› 2002, Vol. 23 ›› Issue (1): 1-5.

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Molecular Cloning of cDNA of Dopamine D₂ Receptor from Human Fetal Brain

Zhao Huanying, Duan Deyi, Zhang Zhi, Xu Qunyuan, Yang Hui

Beijing Institute for Neuroscience and Beijing Center for Neural Regeneration and Repairing, Capital University of Medical Sciences

Received:2001-12-25 Revised:1900-01-01 Online:2002-01-15 Published:2002-01-15

Abstract

Abstract: In order to clone the human gene of dopamine D₂ receptor for treatment of Parkinson disease, total cellular RNA was isolated from human fetal brain and reversely transcribed into cDNA, which was amplified by PCR with the specific primers. The PCR products were inserted into pGEM-T vector for sequencing after emzymatic analysis. The results showed that two fragments were obtained. One, 1 445 bp, was identical to that in the GenBank(NM000795)and the other, 1 305 bp with a deletion of 140 bp, was different from all the three transcripts of D₂ receptors previously described and it may be a novel transcript.

Key words: dopamine receptor, Parkinson disease, gene cloning

CLC Number:

Q781

Zhao Huanying;Duan Deyi;Zhang Zhi;Xu Qunyuan;Yang Hui . Molecular Cloning of cDNA of Dopamine D₂ Receptor from Human Fetal Brain[J]. Journal of Capital Medical University, 2002, 23(1): 1-5.

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Molecular Cloning of cDNA of Dopamine D₂ Receptor from Human Fetal Brain

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