Simultaneous determination of five psychoactive drugs in overdosed patients serum by high performance liquid chromatography

doi:10.3969/j.issn.1006-7795.2013.02.020

Journal of Capital Medical University ›› 2013, Vol. 34 ›› Issue (2): 270-274.doi: 10.3969/j.issn.1006-7795.2013.02.020

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Simultaneous determination of five psychoactive drugs in overdosed patients serum by high performance liquid chromatography

GUO Wei¹, ZHAO Ye¹, GUO Guixin¹, ZHANG Jun¹, SUN Chuan¹, RONG Zhang¹, DUAN Xiaoxia², LI Wenbiao¹, ZHAI Yimin¹

1. Laboratory of Clinical Psychopharmacological, Beijing Anding Hospital, Capital Medical University, Beijing 100088, China;
2. Department of Clinical Chemistry, Psychiatric Hospital of Taiyuan, Taiyuan 030045, Shanxi Province China

Received:2012-08-24 Online:2013-04-21 Published:2013-04-17
Supported by:
This study was supported by Talents Fund of Beijing Municipal Party Committee Organization Department(2004); Capital Medical Research and Development Fund(2009-3135).

Abstract

Abstract:

Objective A high performance liquid chromatography(HPLC) method with ultraviolet(UV) detection(230 nm) was developed and validated for the quantification of 5 drugs, Risperidone, clozapine, reboxetine, 9-hydroxy Risperidone and norclozapine, in human serum. Methods Following a one-step liquid-liquid extraction with diethyl ether, the analyte and internal standard(paroxetine) were separated using an isocratic mobile phase consisted of acetonitrile: methanol: butylamine: acetic acid: deionized water=20: 2.9: 1.1: 1: 34.3 on a reverse phase Inertsil C₁₈-3 column. Five drugs and IS were separated well under the chromatographic condition. The calibration curve was verified over the range of 100~1 600 μg/L and limit of detection(LOD) was 25 μg/L(S/N=10). Results The imprecision of within and between batches were 3.1%~15.6% and 5.7%~18.4%, respectively. The accuracy of within and between batches were 0.1%~8.7% and 0.3%~13.3%. Commonly used psychotropic drugs did not interfere with the drugs and internal standard. Conclusion This method was found to be rapid, simple, accurate, sensitive and more suitable for rapid qualitative and quantitative determination of drugs in the serum of overdosed patients.

Key words: overdose, liquid-liquid extraction, high performance liquid chromatography

CLC Number:

R971⁺.41

GUO Wei, ZHAO Ye, GUO Guixin, ZHANG Jun, SUN Chuan, RONG Zhang, DUAN Xiaoxia, LI Wenbiao, ZHAI Yimin. Simultaneous determination of five psychoactive drugs in overdosed patients serum by high performance liquid chromatography[J]. Journal of Capital Medical University, 2013, 34(2): 270-274.

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Simultaneous determination of five psychoactive drugs in overdosed patients serum by high performance liquid chromatography

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