Hypoxia inducible factor-1α-dependent epithelial to mesenchymal transition under hypoxic conditions in prostate cancer cells

doi:10.3969/j.issn.1006-7795.2014.03.003

Abstract

Abstract:

Objective To explore the influence of hypoxia-inducible factor-1α(HIF-1α) on prostate cancer epithelial to mesenchymal transition(EMT) under hypoxic conditions. Methods In this study we carried out several methods in molecular biology to test whether PC3, a human prostate cancer cell line, underwent typical epithelial to mesenchymal transition under hypoxia and the key regulator of this process. Results We demonstrated that hypoxia induced diverse molecular, phenotypic, and functional changes in prostate cancer cells that are consistent with EMT. We also showed that HIF-1α, which is thought to be stabilized under hypoxic environment, is involved in EMT and cancer cell invasive potency. Hypoxia-induced EMT in prostate cancer cells is blocked by HIF-1α gene silencing. Conclusion EMT may be induced in hypoxic prostate cancer cells, by a mechanism that involves activation of HIF-1α-dependent cell signaling.

Key words: hypoxic environment, hypoxia-inducible factor-1α, prostate cancer, epithelial to mesenchymal transition

CLC Number:

R697

Wang Yongxing, Jiang Yongguang, Luo Yong, Zhao Jiahui, Chen Yatong, Han Yili, Lin Yunhua. Hypoxia inducible factor-1α-dependent epithelial to mesenchymal transition under hypoxic conditions in prostate cancer cells[J]. Journal of Capital Medical University, 2014, 35(3): 278-283.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2014.03.003

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2014/V35/I3/278

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