Radiation suppresses neointimal hyperplasia after revascularization

doi:10.3969/j.issn.1006-7795.2015.01.007

Abstract

Abstract: Objective To study the effect of postoperative fractionated X-ray radiotherapy, at a total dose of 28 Gy, on neointimal formation and strive to further elucidate the mechanisms of neointimal hyperplasia in the abdominal expanded polytetrafluoroethylene (ePTFE) prosthetic vascular graft. Methods The subrenal abdominal aortas of 12 mongrel dogs were replaced with an ePTFE graft. The dogs were randomly divided into either a radiotherapy group or a non-radiotherapy control group. Grafts were harvested at five weeks after surgery for comparing the thickness of neointimal formation and the expression of PCNA, S phase kinase associated protein 2 (Skp2) and p27^kip1 in the two groups.Results We observed that the graft neointimal and anastomotic vessel wall of the radiotherapy group were significantly thinner than those of the control group (P<0.01). The mRNA and the protein expression of p27^kip1 was elevated in the neointimal area after irradiation (P<0.01). The mRNA and the protein expression of PCNA and Skp2 were significantly down-regulated in the radiotherapy group (P<0.01). Conclusion Postoperative fractionated radiotherapy(28 Gy)after an ePTFE graft replacement of the abdominal aorta suppressed the development of neointimal hyperplasia and the proliferation of vascular smooth muscle cell (VSMC) in the graft in the short term.

Key words: expanded polytetrafluoroethylene, radiation, neointimal hyperplasia, Skp2, proliferating cell nuclear antigen, p27^kip1

CLC Number:

R622

Yuan Liqin, Shu Chang, Zhou Xiao, Zhang Wenbo, Fang Kun, Li Xin, Li Quanming. Radiation suppresses neointimal hyperplasia after revascularization[J]. Journal of Capital Medical University, 2015, 36(1): 34-39.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2015.01.007

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2015/V36/I1/34

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