Morphological and inflammatory factor changes of substantia nigra dopaminergic neurons and microglial cells after intraperitoneal injection of lipopolysaccharide in rat

doi:10.3969/j.issn.1006-7795.2015.06.010

Abstract

Abstract: Objective To observe the morphological change of dopaminergic neurons and microglia after intraperitoneal injection of lipopolysaccharide(LPS). Methods Healthy two-month- and one-year-old SD rats were intraperitoneally injected with LPS(1 mg/kg) at 6 h, 12 h, 24 h, 48 h, and 1 w, respectively. Anti tyrosine hydroxylase(TH) and anti ionized calcium binding adapter molecule 1(Iba-1) immunohistochemial staining was utilized to observe TH positive neurons and activity of microglia in substantia nigra. The level of tumor necrosis factor α(TNF-α), interleukin 6(IL-6) and interleukin 1β(IL-1β) in blood and cerebrospinal fluid were detected by enzyme-linked immunosorbent assay(ELISA). Results Microglia of one-year-old SD rats were activated more significantly, and reached the peak earlier than the young group. The activation of microglia was earlier than the damage of dopaminergic neuron in each non-control group. The level of inflammatory factors in aged group was higher than that in the young group in cerebrospinal fluid. Conclusion Aging factors are related with CNS inflammatory reaction which is induced by the peripheral inflammatory media.

Key words: microglial cells, lipopolysaccharide(LPS), dopaminergic neuron, cerebrospinal fluid, tumor necrosis factor α(TNF-α), interleukin 6(IL-6), interleukin 1β(IL-1β)

CLC Number:

R742.5

Bai Lijuan, Ren Yan, Luo Xiaoguang, Jiang Xin, Chen Xiaohong. Morphological and inflammatory factor changes of substantia nigra dopaminergic neurons and microglial cells after intraperitoneal injection of lipopolysaccharide in rat[J]. Journal of Capital Medical University, 2015, 36(6): 887-894.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2015.06.010

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2015/V36/I6/887

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