Effect of intraperitoneal injection of lipopolysaccharides on the substantia nigra NADPH oxidase in rats

doi:10.3969/j.issn.1006-7795.2015.06.009

Abstract

Abstract: Objective To observe the expression of nicotinamide-adenine dinucleotide phosphate(NADPH) oxidase and the production of reactive oxygen species(ROS) in substantia nigra after intraperitoneal injection of lipopolysaccharide(LPS) in rats. Methods Healthy two-month-old and one-year-old SD rats were intraperitoneally injected with LPS(1 mg/kg) at 6 h, 12 h, 24 h, 48 h, and 1 week, respectively. Substantia nigra was taken, reactive oxygen species(ROS) and the activity of NADPH oxidase in the rats were determined. Results At 12 h after intraperitoneal injection with LPS, the activity of ROS in substantia nigra reached the peak and NADPH activity also reached the peak. Conclusion The increase in expression of NADPH oxidase and the shift to membranes protein promotes the inflammatory reaction, however there was no significant differences between the aged group and the young group.

Key words: lipopolysaccharide(LPS), microglia, reactive oxygen species(ROS), NADPH oxidase(PHOX)

CLC Number:

R742.5

Bai Lijuan, Jiang Xin, Chen Xiaohong, Ren Yan, Luo Xiaoguang. Effect of intraperitoneal injection of lipopolysaccharides on the substantia nigra NADPH oxidase in rats[J]. Journal of Capital Medical University, 2015, 36(6): 882-886.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2015.06.009

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2015/V36/I6/882

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