A study on the relationship between ovarian tissue viability and time under normal temperature after thawing

doi:10.3969/j.issn.1006-7795.2019.04.010

Journal of Capital Medical University ›› 2019, Vol. 40 ›› Issue (4): 544-548.doi: 10.3969/j.issn.1006-7795.2019.04.010

• Gynecological Endocrinology and Menopause • Previous Articles Next Articles

A study on the relationship between ovarian tissue viability and time under normal temperature after thawing

Jin Fengyu¹, Ruan Xiangyan^1,2, Alfred O. Mueck^1,2, Du Juan¹, Li Yanglu¹, Cheng Jiaojiao¹, Wang Husheng¹

1. Department of Gynecological Endocrinology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China;
2. Department for Women's Health, University Women's Hospital and Research Center for Women's Health, University of Tuebingen, Tuebingen D-72076, Germany

Received:2019-05-20 Online:2019-07-21 Published:2019-07-19
Supported by:
This study was supported by Nature Science Foundation of Beijing(Y181004), Foundation of Beijing Municipal Science & Technology Commission (Z161100000516143), Beijing Capital's Foundation for Science Development and Research (2016-2-2113), Beijing Municipal Administration of Hospitals' Ascent Plan (DFL20181401), Beijing Municipal Administration of Hospitals Incubating Program (PZ2019031),SAFEA:Project for Key Foreign Experts (20181100005), Beijing Municipality Health Technology High-level Talent (2014-2-016), Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding (XMLX201710).

Abstract

Abstract: Objective To estimate the thawed ovarian tissue viability and explore the feasibility of short distance transportation after thawing different periods at room temperature conditions. Methods Thirteen patients in total,9 samples (2 mm in diameter) were obtained from each patient. One fresh sample was performed follicle counts and the other 8 samples were cryopreservatethawed and randomly divided into 4 groups depends on the time after thawing,that means group 1(0 min), group 2(20 min), group 3(40 min), group 4(60 min). Viable follicle counts,steroid hormone lactic acid and glucose metabolism were performed. Results There were no statistical differences in follicles viability between the fresh group and 4 groups after thawing. And no statistical differences in follicles viability,steroid hormone, lactic acid and glucose metabolism between 4 groups after thawing. Conclusion The ovarian tissue viability did not be impaired after thawing at room temperature in one hour.

Key words: ovarian tissue preservation, fertility protection, thawing, follicle counts

CLC Number:

R711.77

Jin Fengyu, Ruan Xiangyan, Alfred O. Mueck, Du Juan, Li Yanglu, Cheng Jiaojiao, Wang Husheng. A study on the relationship between ovarian tissue viability and time under normal temperature after thawing[J]. Journal of Capital Medical University, 2019, 40(4): 544-548.

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A study on the relationship between ovarian tissue viability and time under normal temperature after thawing

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