M2 macrophage promotes myocardial regeneration by modulating nerve regeneration and angiogenesis in neonatal mice

doi:10.3969/j.issn.1006-7795.2022.02.003

Abstract

Abstract: Objective To determine the effects of polarized macrophages on myocardial regeneration and repair. Methods Macrophages were isolated from rat bone marrow and polarized to M1 or M2 microphage in vitro. Apical resection surgery was performed in neonatal mice on postnatal day 1 or day 7. M1 or M2 macrophages were directly injected into the resection site post resection to determine the effects of two types of polarized macrophages on myocardial regeneration and repair. Animals were sacrificed seven days after surgery to evaluate the myocardial repair with histological methods. Nerve regeneration and angiogenesis were also determined by immunohistochemistry and molecular biology analysis. Results Compared with control animals, M1 macrophage administration inhibited myocardial repair by aggravating inflammatory response. In contrast, M2 macrophage implantation significantly improved myocardial regeneration and repair by alleviating inflammation, stimulating sympathetic innervation and enhancing angiogenesis in damaged myocardial tissue. M2 macrophage implantation also increase the levels of vascular endothelial growth factor(VEGF) and nerve growth factor(NGF), two critical factors for neo-angiogenesis and sympathetic innervation respectively, in damaged area in a dose-dependent manner. Conclusion Our study demonstrates that M2 macrophage promotes myocardial regeneration and repair in vivo, suggesting that modulating macrophage polarization may provide a novel therapeutic strategy to heart disease.

Key words: macrophages, apical resection, myocardial regeneration

CLC Number:

R541

Wang Qian, Zhang Jiakun, Liu Qing, Sun Lin, Li Jingjie. M2 macrophage promotes myocardial regeneration by modulating nerve regeneration and angiogenesis in neonatal mice[J]. Journal of Capital Medical University, 2022, 43(2): 178-186.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2022.02.003

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2022/V43/I2/178

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