Immune characteristics of short-term chronic obstructive pulmonary disease model

doi:10.3969/j.issn.1006-7795.2023.02.004

Abstract

Abstract:

Objective To analyze the immune characteristics of short-term chronic obstructive pulmonary disease (COPD) model induced by peptide challenge after smoking exposure. Methods A short-term murine model of COPD was established by smoking sensitization and peptide infusion challenge. Airway inflammation was analyzed by histological staining sections of lung tissues and cells of bronchoalveolar lavage fluid. An invasive lung function test was used to measure changes of lung respiratory mechanical parameters in experimental mice.The Luminex instrument platform was employed to quantify concentrations of cytokines in lung homogenates. Flow cytometry was used to measure changes in the numbers and proportions of subsets of T helper (Th) cells and innate lymphoid cells (ILCs) in mouse lung tissues. Results The data showed that short-term tobacco smoke exposure and peptide challenge caused damage of the alveoli, induced airways inflammation with neutrophil infiltration, increased the expression of type 3 cytokines and the numbers of Th17 and ILC3, while decreased proportion of ILC1 and ILC2. Conclusion The short-term COPD models of smoke sensitization and peptide challenge are dominated by type 3 immunity, in which ILC3 may play a more important role than Th17.

Key words: chronic obstructive pulmonary disease, cigarette smoke, innate lymphoid cells

CLC Number:

R392

Zhang Hanxiao, Duan Luo, Zhang Muzhi, Zhang Ruoyang, Zhao Liuyinuo, Zhou Ruonan, Li Yunqi, Lyu Zhe, Wang Jingjing, Yuan Huihui, Cui Ye, Sun Ying, Wang Wei. Immune characteristics of short-term chronic obstructive pulmonary disease model[J]. Journal of Capital Medical University, 2023, 44(2): 203-211.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2023.02.004

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2023/V44/I2/203

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