Abstract: Objective  To study the infiltration of neutrophils and macrophages in the tissues of eosinophilic gastroenteritis mice. Methods  Thirty female C57BL/6 mice aged 6-8 weeks were selected to induce the model of eosinophilic gastroenteritis in wild-type mice. They were sensitized by intraperitoneal injection of  ovalbumin （OVA） protein and they were gastric stimulated with OVA. The successful modeling of eosinophilic gastroenteritis was verified by measuring the weight change of mice, detecting OVA specific immunoglobulin E (IgE) and Immunoglobulin G (IgG) levels in peripheral blood of mice by enzyme-linked immunosorbent assay （ELISA）, and detecting intestinal eosinophils count by flow cytometry. The proportion of neutrophils and monocytes in peripheral blood and the proportion of neutrophils and macrophages in spleen, mesenteric lymph nodes, small intestine and colon tissues were detected by flow cytometry. Results  The mice model of eosinophilic gastroenteritis was successfully induced by OVA abdominal sensitization and gavage stimulation. In mice with eosinophilic gastroenteritis, the infiltration of monocytes in peripheral blood, macrophages infiltration of spleen, mesenteric lymph nodes, small intestine and colon tissues were increased. And the neutrophil were increased infiltrated in peripheral blood, small intestine and colon tissues. Conclusion  Macrophage and neutrophil were increased infiltrated in peripheral blood, intestinal tissue of mice with eosinophilic gastroenteritis. Furthermore, the infiltration of macrophage was increased in lymphoid tissues, such as spleen and mesenteric lymph nodes of mice with eosinophilic gastroenteritis.

Key words: mice, eosinophilic gastroenteritis, eosinophils, neutrophils, macrophages