Abstract:

The aim was to study the expression of signal transduction related genes on huma n cerebral aneurysm s with microarray gene chip. 8 464 genes of the PCR products were spotted onto a glass plates in array. DNAs were fixed onto the glass plate. The total RNAs wer e isolated from the tissue, and were purified to mRNAs by Oligotex. Both mRNAs f rom the 4 human cerebral aneurysms and normal human Willis circle arteries were reversely transcribed to cDNAs with hybridization probes using the incorporation of Cy3-dCTP and Cy5-dCTP. The mixed probes were hybridized to the cDNA micr oarray. The cDNA microarray was scanned for the fluorescent signals and showed d ifferences between aneurysm and normal control. Among the 8 464 target genes, 15 signal transduction related genes showed significant different expression betw een aneurysms and normal control with 13 genes upregulated and 2 genes downregul ated. The differentially expressed genes and related pathology can play a key role in the pathogenesis of cerebral aneurysms.

Key words: cerebral aneurysm, signal transducti on, microarray