Journal of Capital Medical University ›› 2007, Vol. 28 ›› Issue (2): 173-175.

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Resistant Genes Detection and Homologous Analysis ofVancomycin Resistant Enterococcus

Liu Zhiyuan, Xu Shuzhen, Yu Yanhua   

  1. Clinical Laboratory Center, Beijing Friendship Hospital, Capital Medical University
  • Received:2007-01-16 Revised:1900-01-01 Online:2007-04-24 Published:2007-04-24

Abstract:

Objective To detect vancomycin-resistant genes of enterococcus, and to analyses antibiotic resistant phenotype and genotype. Methods Susceptibility of vancomycin and other antibiotics were performed with agar diffusion methods. Vancomycin resistant genes and homologous analysis were detected with PCR and RAPD methods, respectively. Results 6 strains of Enterococcus gallinarum and 4 of Enterococcus faecium were identified in the study, all of which carried vanA gene. According to RAPD results, 10 strains were divided into 7 genetypes. Conclusion Vancomycin resistance in enterococcus is mainly mediated by vanA. RAPD is the effective methods to analyze the homogeneity of vancomycin resistant enterococcus.

Key words: vancomycin, enterococcus, drugresistance, microbe, gene

CLC Number: