关键词: 糖化白蛋白, 近端肾小管上皮细胞, 肾损伤分子-1, 中性粒细胞明胶酶相关载脂蛋白, 细胞因子

Abstract: Objective To investigate the effects of glycated albumin (GA) on the expression of kidney injury molecule-1 (KIM-1), neutrophil gelatinase-associated lipocalin (NGAL) and inflammatory cytokines in human proximal tubular epithelial cells (HK-2 cells). Methods The HK-2 cells were treated with the different concentrations of GA and albumin (Alb) for 12h, 24h and 48h. The mRNA expressions of KIM-1 and NGAL were detected by real-time PCR. The release of KIM-1 and NGAL in the supernatants were detected by ELISA. The concentrations of VEGF, TNF-α, IL-8 and sICAM-1 were detected by cytometric beads array method. Results Compared with control and Alb groups, the mRNA levels of KIM-1 and NGAL in HK-2 cells were significantly up-regulated at 12 h, 24 h and 48 h after GA treatment (P<0.05); the protein levels of KIM-1 and NGAL released in supernatants of GA-treated cells were significantly higher than those in control group and Alb groups at the same time points (P<0.05); GA groups also had significantly higher levels of sICAM-1, VEGF and IL-8 than control group at each time point (P<0.05). Conclusion GA can up-regulate the expression and release of KIM-1 and NGAL and promote the secretion of inflammatory cytokines which could cause damage to renal tubules, suggesting that GA may reflect the diabetic renal involvement.

Key words: glycated albumin, proximal tubular epithelial cell, kidney injury molecule-1, neutrophil gelatinase-associated lipocalin, cytokines