Abstract: Objective To probe into the mechanism of Hou Shi Hei San in accelerating repair of nerve after cerebral ischemia in rats by observing the changes of growth-associated protein-43(GAP-43), microtubule-associated protein-2(MAP-2) and synatophysin(SYN) in the tissue of cerebral ischemia. Methods Middle cerebral artery occlusion model was replicated by string ligation. Using behavioral test, the authors valuated the neurological deficiency of each group after operation. The injury and repair of neuron, dentrite and axon were observed by immunohistochemistry combined with image pattern analysis 15 days after the rat models were made. Results There were obvious disturbances of nerve function, scarce dentrite of MAP-2 positive cell and immunoreaction of SYN reduced in the model group. The grade of injuries of nerve function in the group treated with Hou Shi Hei San was lower than that in the model group(P<0.05). The area and intensity of expression of GAP-43 in CAI and CA3 areas of hippocampi in treatment groups were significantly higher than that in model group(P<0.05). The integrated optical density(IOD) values of MAP-2 and SYN expressions markedly increased in treatment groups(P<0.05). Conclusion These results suggest that Hou Shi Hei San can increase the expressions of GAP-43, MAP-2 and SYN and promotes nerve regeneration through inducing the synthesis of construction protein of neuron effectively, thus accelerates the repair of nerve.

Key words: Hou Shi Hei San, cerebral ischemia, repair of nerve