Journal of Capital Medical University ›› 2009, Vol. 30 ›› Issue (3): 336-340.doi: 10.3785/j.issn.1006-7795.2009.03.017

• 基础研究 • Previous Articles     Next Articles

Study on Method for Determination of Perfluorooctane Sulfonate Concentration in Serum Using High Performance Liquid Chromatography-Electrospray Tandem Mass Spectrometry

ZHAO Yi-song1, CUI Xin2, LIANG Kun2, LIU Shuang2, YANG Lin2, ZHAO Feng3   

  1. 1. Chemical Biology and Pharmaceutical Laboratory, School of Chemical Biology and Phamaceutical Sciences, Capital Medical University;2. Department of Preventive Medicine, Capital Medical University;3. Department of Occupational and Environment Health, School of Public Health and Family Medicine, Capital Medical University
  • Received:2007-11-27 Revised:1900-01-01 Online:2009-06-21 Published:2009-06-21

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Abstract: Objective To develop an analytical method for the determination of perfluorooctane sulfonate(PFOS). Methods In the procedure a solid-phase extraction for the isolation of PFOS was employed. PFOS was subsequently analyzed by high performance liquid chromatography coupled with electrospray tandem mass spectrometry internal standard method. Mass spectral acquisition was applied with multiple reaction monitoring(MRM). Results The calibration curve was linear over a range from 0 μg/L to 120 μg/L with a correlation coefficient of 0.991 039. The average recovery for PFOS was 76.88%. Conclusion The method enables the precise determination of standards and can be applied to detect PFOS in human plasma samples for monitoring human exposure.

Key words: high performance liquid chromatography, electrospray tandem mass spectrometry, perfluorooctane sulfonate

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