Establishment of a Novel Co-culture Model of Suprachiasmatic Nucleus Slices and NIH/3T3 Cells in a Serum-free Condition

doi:10.3969/j.issn.1006-7795.2009.05.007

Journal of Capital Medical University ›› 2009, Vol. 30 ›› Issue (5): 601-606.doi: 10.3969/j.issn.1006-7795.2009.05.007

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Establishment of a Novel Co-culture Model of Suprachiasmatic Nucleus Slices and NIH/3T3 Cells in a Serum-free Condition

ZUO Xiao-hong¹, CAI Yan-ning¹, LI Ning^1,2, YU Shun¹, ZHANG Yan-li¹, CHEN Biao¹

1. Department of Neurobiology, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University; Key Laboratory of Neurodegenerative Diseases of Ministry of Education;2. Laboratory of Cell and Development, College of Life Sciences, Capital Normal University

Received:2009-07-16 Revised:1900-01-01 Online:2009-10-21 Published:2009-10-21

Abstract

Abstract: Objective To establish a novel co-cultures model of suprachiasmatic nucleus(SCN) slices and NIH/3T3 cells in a serum-free condition. Methods SCN or cortical slices prepared from 7-day-old male SD rats were cultured with NIH/3T3 cells for 6 d. 24 h expression profile of Per1 was examined in NIH/3T3 cells using real-time PCR. Results A significant daily oscillation of Per1 mRNA expression was observed in NIH/3T3 cells co-cultured with SCN slices. The peak time of Per1 was at CT11. No daily oscillation was found in NIH/3T3 cells induced with cortical slices. Conclusion A novel co-culture model of SCN slices and NIH/3T3 cells was established, which will facilitate the studies on the communications between SCN and peripheral tissues.

Key words: circadian, expression, hypothalamic suprachiasmatic nucleus, Per1

CLC Number:

Q 344⁺.1

ZUO Xiao-hong;CAI Yan-ning;LI Ning;YU Shun;ZHANG Yan-li;CHEN Biao. Establishment of a Novel Co-culture Model of Suprachiasmatic Nucleus Slices and NIH/3T3 Cells in a Serum-free Condition[J]. Journal of Capital Medical University, 2009, 30(5): 601-606.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2009.05.007

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2009/V30/I5/601

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Establishment of a Novel Co-culture Model of Suprachiasmatic Nucleus Slices and NIH/3T3 Cells in a Serum-free Condition

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