Abstract: Objective To compare two methods of myelin staining for detecting the morphological changes in the brain of Cuprizone-induced demyelination model mice. Methods Demyelination model was induced by Cuprizone which was mixed in forage and fed to mice for 4 weeks. After the brain tissue sections were made, Luxol Fast Blue(LFB) and oil red O staining methods were used to observe demyelinative changes in the corpus callosum on striatum and hippocampus sections. Results The results from both LFB and oil red O staining showed that demyelination occurred in the corpus callosum of Cuprizone-induced model mice, but the morphological changes in the striatum section were clearer detected by oil red O staining than that measured by LFB staining, and the margin of demyelination with the surrounding tissue was not clear on the section stained by LFB. Conclusion Compared with the LFB staining, Oil red O staining showed clearer demyelination and is a simple myelin staining method.

Key words: Cuprizone, demyelination, oil red O staining, Luxol Fast Blue staining