Local mild hypothermia influences the expression of matrix metalloproteinase 9 and extracellular matrix proteins in a rat model of ischemia and reperfusion injury

doi:10.3969/j.issn.1006-7795.2013.01.011

Abstract

Abstract:

Objective To investigate the effects of local mild hypothermia on expression of matrix metalloproteinase 9 (MMP9), extracellular matrix proteins laminin (LN) and fibronectin (FN) in a rat model of ischemia/reperfusion(I/R) injury. Methods Ischemia and reperfusion injury was produced in Sprague Dawley rats with modified suture-occlusion method. The ischemia caused by suture was persisted for two hours in the bodies of rat I/R injury models then the suture was removed. These I/R injury models were randomly assigned into the normothermia group and hypothermia group. Ice was placed under the heads of rats of the local hypothermia group and the cooling lasted for 1 h after reperfusion. Groups of normothermia and hypothermia were further divided into six subgroups with 4 h (N-4 h group and H-4 h group), 24 h (N-24 h group and H-24 h group), 72 h (N-72 h group and H-72 h group) of reperfusion, respectively. 2,3,5-triphenyltetrazolium chloride (TTC) staining was applied to measure the cerebral infarction volume, and the neurological deficits scores stood for the neurological function in N-24 h group and H-24 h group. Expression of MMP9, LN and FN were detected with immunofluorescence in ischemic penumbra of all groups. Results The percentage of cerebral infarction volume of H-24 h group was less than that of N-24 h group (P<0.01). Compared with N-24 h group, the neurological deficits scores of H-24 h group declined significantly (P<0.01). The expression of LN and FN of H-4 h group significantly increased versus N-4 h group (P<0.05). MMP9 reduced in the groups of H-4 h and H-24 h when matched with groups of N-4 h and N-24 h, respectively (P<0.05). ConclusionLocal mild hypothermia can significantly reduce the cerebral infarct volume and ameliorate the neurological dysfunction in rat I/R injury models. And it can diminish the expression of MMP9 and attenuate the loss of LN and FN. These findings indicated that MMP9, LN and FN may be involved in the protective mechanisms of the local hypothermia against cerebral ischemia/reperfusion injury.

Key words: cerebral ischemia/reperfusion, mild hypothermia, matrix metalloproteinase 9, laminin, fibronectin

CLC Number:

R743.31

LI Fang, LIU Xiangrong, YAN Feng, ZHANG Chencheng, LUO Yumin, FANG Boyan, JI Xunming. Local mild hypothermia influences the expression of matrix metalloproteinase 9 and extracellular matrix proteins in a rat model of ischemia and reperfusion injury[J]. Journal of Capital Medical University, 2013, 34(1): 58-63.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2013.01.011

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2013/V34/I1/58

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