Changes in cerebral blood flow and infarct volume in different sexes of rats after focal brain ischemia

doi:10.3969/j.issn.1006-7795.2013.01.012

Abstract

Abstract:

Objective To study the infarct volumes and the cortical blood flow of the rat model of middle cerebral artery occlusion (MCAO) in different sexes.Methods Twenty-four healthy sprague dawley (SD) rats, including 12 males and 12 females, weighing 280-310 g were studied. The rats were subjected to unilateral middle cerebral artery occlusion (MCAO) for 2 hours and sacrificed after 24 hours reperfusion. The cortical blood flow was continuously monitored by laser Doppler flowmetry (LDF). The base line value, the decreasing amplitude after occlusion and the recovering amplitude after reperfusion of the local blood flow were recorded. And the data of the two groups were compared. Brain slices were obtained at the time of ischemia for 2 hours and reperfusion for 24 hours. The volumes of infarction in the brain slices were determined by triphenyltetrazolium chloride (TTC) staining.Results The average baseline value of cortical blood flow of the females and males were (154±20)PU and (212±24)PU separately. The value of the female was significantly lower than that of the male (P<0.01). The baseline value remarkably decreased to (48±3)PU and (78±21)PU separately after occlusion (P<0.05). The decline in the percentage of females was (68±4)%, and that of males was (63±12)%. There was no significant difference in decreasing amplitude of the blood flow between the different sexes(P>0.05). In the reperfusion period, the average local blood flow rapidly recovered to (159±5)PU and (196±36)PU separately. Recovery percentage of females was(104±11)%, and that of the males was(91±14)%. There was no significant difference in recovering amplitude of the blood flow between the different sexes(P>0.05). The infarct volumes of the female group measured by TTC were significantly smaller than that of the male (P<0.05).Conclusion A steady model of middle cerebral artery occlusion in different sexes of rats was established, and it was found that under the normal physical conditions the the average baseline value of cortical blood flow of the females are significantly lower than that of the males. There are no significant differences in both the decreasing amplitude after occlusion and the recovering amplitude after reperfusion between females and males. However the recovery of the blood flow of the females was better than that of the males. Therefore it is supposed that low-level state of CBF may contribute to the tolerance of female rats to the ischemia-reperfusion injury, and at last improved the neurological functions and decreased the infarct volumes.

Key words: rats, sex, middle cerebral artery occlusion, cerebral blood flow

CLC Number:

R743.31

YAN Feng, LIU Xiangrong, ZHAO Haiping, WANG Rongliang, JI Xunming, ZHAO Yongmei, TAO Zhen, LUO Yumin. Changes in cerebral blood flow and infarct volume in different sexes of rats after focal brain ischemia[J]. Journal of Capital Medical University, 2013, 34(1): 64-68.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2013.01.012

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2013/V34/I1/64

References

[1] 夏海波, 张英谦. 雌激素与缺血性脑卒中关系研究进展[J]. 中华老年心血管病杂志, 2012, 14(4):444-446.

[2] 刘照寒, 曲松滨. 雌激素与女性缺血性脑卒中[J]. 现代生物医学进展, 2012, 1(12):195-197.

[3] Kooperberg C, Cushman M, Hsia J, et al. Can biomarkers identify women at increased stroke risk? The Women's Health Initiative Hormone Trials [J]. PLoS Clin Trials, 2007, 2(6): e28.

[4] Lisabeth L D, Beiser A S, Brown D L, et al. Age at natural menopause and risk of ischemic stroke: the framingham heart study [J]. Stroke, 2009, 40(4): 1044-1049.

[5] Alkyaed N J, Harukunil, Kimes A S, et al. Genderlikned brain injury inexperimented stroke [J]. Stroke, 1998, 29(1): 159-166.

[6] Simpkins J W, Rajakuma G, Zhang Y Q, et al. Esortgens may reduce mortality and ischemic damage caused by middle cerebral artery occlusion in the female rat [J]. J Neurosurg, 1997, 87(5): 724-730.

[7] Toung T J, Tragsmtna R J, Hurn P D. Esrtogen-mediated neuroprotection after experimented sortke in male rats[J]. Stroke, 1998, 29(8): 1666-1670.

[8] 吴乐, 黎红华, 陈文军, 等. 雌激素对绝经后雌性大鼠缺血性脑损伤的保护作用及机制[J]. 卒中与神经疾病, 2008, 15(1): 6-8.

[9] Liao S, Chen W, Kuo J, et al. Association of serum estrogen level and ischemic neuroprotection in female rats [J]. Neuorsci Lett, 2001, 297(3): 159-162.

[10] McCullough L D, Alkayed N J, Traystman R J, et al. Postischemic estrogen reduces hypoperfusion and secondary ischemia after experimental [J]. Stroke, 2001, 32(3): 796-803.

[11] Strom J O, Theodorsson E, Holm L, et al. Different methods for administering 17b-estradiol to ovariectomized rats result in opposite effects on ischemic brain damage [J]. BMC Neuroscience,2010, 39(5):1407-1415.

[12] Ardelt A A, Anjum N, Rajneesh K F, et al. Estradiol augments peri-infarct cerebral vascular density in experimental stroke [J]. Exp Neurol, 2007, 206(1): 95-100.

[13] Hurn P D, Little-Kearney M T, Kirsch J R, et al. Postischemic cerebral blood flow recovery in the female: effect of 17 beta-estradiol [J]. J Cereb Blood Flow Metab, 1995, 15(4): 666-672.

[14] Vitale C, Mercuro G, Cerquetani E, et al. Time since menopause influences the acute and chronic effect of estrogens on endothelial function [J]. Arterioscler Thromb Vasc Biol, 2008, 28(2): 348-352.

[15] Dhandapani K M, Wade F M, Mahesh V B, et al. Astrocyte-derived transforming growth factor-{beta} mediates the neuroprotective effects of 17{beta}-estrogen: involvement of nonclassical genomic signaling pathways [J]. Endocrinology, 2005, 146(6): 2749-2759.

[16] 于海燕, 宫殿荣, 陈德哲. 雄激素与缺血性脑血管病[J]. 国际脑血管病杂志, 2010, 18(10): 773-776.