Neuroprotective effect of R(+)-pramipexole on brain injury following transient focal cerebral ischemia in rats

doi:10.3969/j.issn.1006-7795.2014.02.017

Abstract

Abstract:

Objective To study the effect of R(+)-pramipexole on reactive oxygen species(ROS) production and brain injury in transient focal ischemic rats and to investigate the neuroprotective effects of R(+)-PPX on cerebral ischemic injury and explore the preventive and therapeutic methods for ischemic cerebrovascular disease. Methods Thirty male Sprague Dawley rats were divided randomly into three groups: Sham group(n=10), middle cerebral artery occlusion group(MCAO, n=10) and MCAO+ R(+)-PPX group. MCAO operation was performed by using suture method. The rats underwent right MCAO for 90 min. The heart rate, mean arterial blood pressure(MABP) and rectal temperature were monitored to keep in normal range during the operation. At 6 h or 24 h after reperfusion, brains were removed and dyed with 2, 3, 5-triphenyltetrazolium chloride to measure the infarct volume. H2DCF-DA was used to detect ROS in the penumbra of the brain. Results 1) There was no statistical difference in heart rates, MABP and rectal temperature among sham, MCAO and MCAO+R(+)-PPX group rats. 2) No infarction was observed in the brain of sham group rats whereas the brain infarct volume in both MCAO group and MCAO+ R(+)-PPX group increased significantly at 6 h to 24 h after reperfusion(P<0.05). The infarct volume of both MCAO group and MCAO+R(+)-PPX group showed time-dependent increasing tendency(P<0.05). At 6 h after reperfusion, the brain infarct volume in MCAO+R(+)-PPX group rats decreased significantly compared with that of MCAO group rats(P<0.05) while the brain infarct volume in MCAO+ R(+)-PPX group rats showed no statistical difference compared with that of MCAO group rats at 24 h after reperfusion(P>0.05). 3) No penumbra was observed in the brain of sham group rats. The number and fluorescence intensity of H2DCF-DA-positive cells in the penumbra of MCAO group and MCAO+R(+)-PPX group rats from 6 h to 24 h after reperfusion showed time-dependent increasing tendency(P<0.05). At 6 h after reperfusion, the number of H2DCF-DA-positive cells and fluorescence intensity in the penumbra of MCAO+R(+)-PPX group rats decreased significantly compared with that of MCAO group rats(P<0.05) while the number of H2DCF-DA-positive cells and fluorescence intensity in the penumbra of MCAO+R(+)-PPX group rats showed no statistical difference with that of MCAO group rats at 24 h after reperfusion(P>0.05). Conclusion A single administration of R(+)-PPX could decrease ROS production, reduce infarct volume in MCAO rats at early reperfusion time(6 h). The effect of R(+)-PPX on cerebral ischemic injury deserves further study.

Key words: cerebral ischemia, middle cerebral artery occlusion, reperfusion, R(+)-PPX, rats

CLC Number:

R743.31

Yin Jie, Yan Feng, Luo Yumin, Li Sen, Liu Kejian, Zhao Yongmei. Neuroprotective effect of R(+)-pramipexole on brain injury following transient focal cerebral ischemia in rats[J]. Journal of Capital Medical University, 2014, 35(2): 225-230.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2014.02.017

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2014/V35/I2/225

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