Differences of liver CD4+CD25+Foxp3+ regulatory T cells in mice with acute and chronic liver injury

doi:10.3969/j.issn.1006-7795.2014.04.020

Abstract

Abstract:

Objective The present study focuses on the differences and significance of intrahepatic CD4+CD25+Foxp3+ regulatory T cells(Tregs) between acute and chronic liver injury. Methods C57BL/6J mice were treated with carbon tetrachloride(CCL₄) by intraperitoneal injection, to prepare acute(24 h) and chronic liver injury(6 weeks) model; control mice were injected with saline. The pathological changes of livers in different mouse models were observed by Masson staining; the expression of intrahepatic Tregs was analyzed by flow cytometry and immunofluorescence staining; the mRNA levels of collagen-Ⅰ, collagen-Ⅲ, IL-1β、IL-6、IL-10、TGF-β were detected by real-time PCR. Results Masson staining showed a large liver cell necrosis with bleeding in acute liver injury mice; however, pseudolobule formed in the liver of chronic liver injury mice, while type-Ⅰ, type-Ⅲ collagen expression was increased. Tregs expression has elevated in both acute and chronic liver injury mice, but chronic injury group increased in magnitude larger than acute injury group(P<0.01). Intrahepatic inhibitory cytokine IL-10, TGF-β in chronic liver injury group were increased larger than that in acute injury liver group(P<0.05); while the pro-inflammatory cytokines IL-1β, IL-6 in mice with acute liver injury were enhanced more significantly comparing to mice in chronic liver injury(P<0.01). Conclusion The expression of intrahepatic Tregs was different between acute and chronic liver injury. In acute liver injury, especially in the recovery of acute liver injury, Tregs increased in part to inhibit the proliferation of inflammation, and promote the body's recovery; in chronic liver injury, Tregs were significantly increased to induce formation of immune tolerance, suppress immune clearance, finally led to hepatic fibrosis.

Key words: regulatory T cells, acute liver injury, chronic liver injury, immune suppression

CLC Number:

R575

Zhang Xiaohui, Liu Xin, Bai Li, Chen Yu, Duan Zhongping. Differences of liver CD4+CD25+Foxp3+ regulatory T cells in mice with acute and chronic liver injury[J]. Journal of Capital Medical University, 2014, 35(4): 483-487.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2014.04.020

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2014/V35/I4/483

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