Change of G-protein expression in the myocardial damage induced by acute brain injury

doi:10.3969/j.issn.1006-7795.2015.01.021

Abstract

Abstract: Objective To test the effect of G_s-protein and G_q-protein on cardiac damage induced by acute brain injury, and the contribution of β1-ARB and 5-HT_2ARB to G-protein. Methods The Wistar rat model of acute brain injury was built. The mRNA level of G_sα and G_qα were evaluated with real time PCR, and the protein level of G_sα and G_qα were detected by Western blotting assay.Results Compared with NORM group, the mRNA level of G_sα was (92.6±24)% in SHAM group, (39.7±30)% in ABI group and (80.1±12)% in BETA group, respectively. The mRNA level of G_sα was significantly decreased in ABI group compared to SHAM group, but significantly increased in β1-ARB treated BETA group, and there is no difference of G_sα mRNA level between β1-ARB treated BETA and SHAM groups. Compared with NORM group, the protein level of G_qα was (105±30)% in SHAM group, (110±14)% in ABI group and (107±23)% in BETA group. There was no statistical difference among three groups. Conclusion The mRNA and protein of G_sα were significantly decreased after acute brain injury, which indicates the occurrence of myocardial damage induced by acute brain injury may be related to the inhibition in signal transduction pathway mediated G_s-protein. G_s-protein may be in the form of expression change involved in the occurrence of myocardial damage induced by acute brain injury.

Key words: brain injury, myocardial damage, 5-HT_2ARB, G_sα-protein, G_qα-protein

CLC Number:

Guo Caixia, Zeng Xiangjun, Du Fenghe, Chen Buxing. Change of G-protein expression in the myocardial damage induced by acute brain injury[J]. Journal of Capital Medical University, 2015, 36(1): 111-115.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2015.01.021

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2015/V36/I1/111

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