Effects of exogenous hydrogen sulfide on autophagy of HepG2 cells

doi:10.3969/j.issn.1006-7795.2015.01.022

Abstract

Abstract: Objective To investigate the effects of exogenous hydrogen sulfide (H₂S) on the autophagy of HepG2 cells and its underlying mechanism.Methods HepG2 cells were administrated by sodium hydrosulfide (NaHS), a donor of H₂S, for 24h, the expression of autophagy-related protein LC3-Ⅱ was detected via Western blotting; The mRNA level of autophagy related gene beclin1 and atg5 were detected via RT-PCR. The autophagy particles and apoptosis were observed using immunofluorescence. AnnexinV/PI flow cytometry were performed to assess the effect of NaHS on cell apoptosis. Results NaHS enhanced the autophagy-related protein (LC3-Ⅱ) expression and beclin1 mRNA, atg5 mRNA and autophagosome formation compared with control. 3-MA could inhibit NaHS-induced autophagy.Immunofluorescence showed the M30 positive cells were significantly increased compared with the controls.Additionally, both the nuclear heterochromatin and apoptotic bodies were significantly increased. Flowcytometry showed that NaHS increased the apoptosis rate of HepG2 cells. Conclusion The autophagy is induced by exogenous hydrogen sulfide through the autophagy related genes (beclin1 and atg5), and can promote apoptosis in HepG2 cells.

Key words: hydrogen sulfide, autophagy, microtubule-associated light chain 3-Ⅱ(LC3-Ⅱ), apoptosis

CLC Number:

R969.9

Chen Ning, Wang Shanshan, Chen Yuhan, Yin Jiming, Chen Dexi, Ding Huiguo. Effects of exogenous hydrogen sulfide on autophagy of HepG2 cells[J]. Journal of Capital Medical University, 2015, 36(1): 116-120.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2015.01.022

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2015/V36/I1/116

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