Relationship between carbonic anhydrase-IX and high risk human papilloma virus infection in cervical exfoliated cells

doi:10.3969/j.issn.1006-7795.2015.02.012

Abstract

Abstract: Objective To explore the value of carbonic anhydrase-IX(CA-IX) expressed in cervical exfoliated cells for predicting the natural outcome of cervical intraepithelial neoplasia(CIN)and analysis the relationship between high risk human papillomavirus(HR-HPV) infection and CA-IX. Methods Totally 333 cases who underwent cervical exfoliated cells cytologic examination through thinprep cytologic test(TCT) in Chaoyang hospital from May 2012 to Dec 2013 were enrolled into this study. Immunocytochemistry and HPV DNA tests were applied to study the relationship between HR-HPV infection and CA-IX. Results There was a correlation between CA-IX and HR-HPV infection, the staining of CA-IX in different HPV-infected cell was 60.6% in persistent HR-HPV infection, 38.4% in transient HR-HPV-infected cells and 15.6% in non-HR-HPV-infected cells. Conclusion There was a correlation between CA-IX and HR-HPV infection, the expression of CA-IX in persistent HR-HPV infection was much higher than that in transient HR-HPV-infected and non-HR-HPV-infected cells, showing that CA-IX may be one of the biomarkers of persistent HR-HPV infection leading to CIN.

Key words: cervical intraepithelial neoplasia, carbonic anhydrase-IX, high risk human papilloma virus(HR-HPV), persistent HR-HPV infection

CLC Number:

R711.74

Liu Yingqiao, He Xin, Liu Jun, Wang Shuzhen, Xu Shasha, Wang Yue, Diao Xiaoli. Relationship between carbonic anhydrase-IX and high risk human papilloma virus infection in cervical exfoliated cells[J]. Journal of Capital Medical University, 2015, 36(2): 226-231.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2015.02.012

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2015/V36/I2/226

References

[1] Shin H J, Rho S B, Jung D C, et al. Carbonic anhydrase IX(CA-IX) modulates tumor-associated cell migration and invasion[J]. J Cell Sci, 2011,124(Pt 7):1077-1087.
[2] Paul P, Rajendran S K, Peuhu E, et al. Novel action modality of the diterpenoid anisomelic acid causes depletion of E6 and E7 viral oncoproteins in HPV-transformed cervical carcinoma cells[J]. Biochem Pharmacol, 2014,89(2):171-184. doi:10.1016.
[3] Nakamura M, Bodily J M, Beglin M, et al. Hypoxia-specific stabilization of HIF-1alpha by human papillomaviruses[J]. Virology, 2009,387(2):442-448.
[4] 黄受方.新版《子宫颈细胞学Bethesda报告系统》读后感[J].诊断病理学杂志,2005,12(3):161-163.
[5] Tristao W, Ribeiro R M, Oliveira C A, et al. Epidemiological study of HPV in oral mucosa through PCR[J]. Braz J Otorhinolaryngol, 2012,78(4):66-70.
[6] Belinson J, Qiao Y, Pretorius R, et al. Prevalence of cervical cancer and feasibility of screening in rural China:a pilot study for the Shanxi Province Cervical Cancer Screening Study[J]. Int J Gynecol Cancer, 1999,9(5):411-417.
[7] Pastorekova S, Zavadova Z, Kost al M, et al. A novel quasi-viral agent, MaTu, is a two-component system[J]. Virology, 1992,187(2):620-626.
[8] Liao S Y, Rodgers W H, Kauderer J, et al. Carbonic anhydrase IX(CA-IX) and high-risk human papillomavirus(H-HPV) as diagnostic biomarkers of cervical dysplasia/neoplasia in Japanese women with a cytologic diagnosis of atypical glandular cells(AGC):a Gynecologic Oncology Group(GOG) Study[J]. Br J Cancer, 2011,104(2):353-360.
[9] Schlecht N F, Kulaga S, Robitaille J, et al. Persistent human papillomavirus infection as a predictor of cervical intraepithelial neoplasia[J]. JAMA, 2001,286(24):3106-3114.
[10] Pastorekova S, Zavadova Z, Kostal M, et al. A novel quasi-viral agent, MaTu, is a two-component system[J]. Virology, 1992,187(2):620-626.
[11] Supuran C T. Carbonic anhydrases:novel therapeutic applications for inhibitors and activators[J]. Nat Rev Drug Discov, 2008,7(2):168-181.
[12] Pastorekova S, Ratcliffe P J, Pastorek J. Molecular mechanisms of carbonic anhydrase IX-mediated pH regulation under hypoxia[J]. BJU Int, 2008,101(Suppl 4):8-15.
[13] Kajo K, Turcan I, Vallova M, et al. Expression of carbonic anhydrase IX in the breast carcinomas[J]. Ceska Gynekol, 2013,78(3):263-268.
[14] Ilardi G, Zambrano N, Merolla F, et al. Histopathological determinants of tumor resistance:a special look to the immunohistochemical expression of carbonic anhydrase IX in human cancers[J]. Curr Med Chem, 2014,21(14):1569-1582.
[15] 刘军,张震宇,王淑珍,等.MN/CAⅨ基因在宫颈上皮内瘤变及宫颈鳞癌中的表达[J].肿瘤预防与治疗,2008,21(4):358-361.
[16] Koshiol J, Lindsay L, Pimenta J M, et al. Persistent human papillomavirus infection and cervical neoplasia:a systematic review and meta-analysis[J]. Am J Epidemiol, 2008,168(2):123-137.
[17] 钱德英,岑坚敏,王丁,等.高危型人乳头状瘤病毒DNA检测与细胞学联合检查对子宫颈癌前病变筛查的研究[J].中华妇产科杂志,2006,41(1):34-37.
[18] 邓永丽,张力,徐宏伟,等.188例宫颈病变患者HPV分型检测的结果分析[J].西南国防医药,2010,20(12):1330-1332.
[19] 窦燕,郭以河,孟加榕,等.漳州地区200例女性宫颈病变HPV感染基因型分析[J].解放军医药杂志,2014,26(8):96-98.
[20] 袁林,徐又先,陈静,等.高危型HPV感染者负性生活事件暴露与宫颈癌发生相关性分析[J].中华肿瘤防治杂志,2013,20(19):1465-1468.