Ceramide induces autophagic cell death in glioma cell through JNK-c-Jun pathway

doi:10.3969/j.issn.1006-7795.2015.02.020

Abstract

Abstract:

Objective To observe the autophagy of glioma cell induced by ceramide and explore the possible mechanism. Methods The viability of C6 cells was measured by MTT assay. The cell apoptosis was assayed by flow cytometry. Autophagic-related protein expressions of LC3B/LC3A and Beclin-1 were determined by Western blotting. The level of JNK-c-Jun induced by ceramide was measured by Western blotting with or without the treatment of JNK specific inhibitor SP600125. Results After treatment of ceramide for 24 hours, the growth of C6 cells were significantly inhibited in dose-dependent manner(P<0.05); and ceramide increased autophagic cell death also in dose-dependent manner(P<0.05). Compared with the control group, the levels of LC3B/LC3A and Beclin-1 were significantly increased in ceramide treatment group(P<0.05). JNK was activated in the C6 cells exposed to ceramide and the phosphorylation of c-Jun also increased. This activation of autophagy could be reversed by the pre-treatment of SP600125. Conclusion Ceramide may induce autophagy in glioma cell and the mechanism may be related to the activation of JNK-c-Jun signaling pathway.

Key words: glioma cell, ceramide, autophagic cell death, JNK signaling pathway

CLC Number:

R739.41

Zhang Luyong, Luo Feiya, Hu Peili, Shan Chun, Zhang Miao. Ceramide induces autophagic cell death in glioma cell through JNK-c-Jun pathway[J]. Journal of Capital Medical University, 2015, 36(2): 276-281.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2015.02.020

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2015/V36/I2/276

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