Effect of EX527 on the protection of SH-SY5Y by calorie restriction

doi:10.3969/j.issn.1006-7795.2015.05.008

Journal of Capital Medical University ›› 2015, Vol. 36 ›› Issue (5): 705-708.doi: 10.3969/j.issn.1006-7795.2015.05.008

Effect of EX527 on the protection of SH-SY5Y by calorie restriction

Zhang Jingshuang^1,2, Ma Lina^1,2, Wang Rong^1,2,3,4, Zhang Jingyan^1,2,4, Wu Yanchuan^1,2,4, Zhao Zhiwei^1,2,4, Wang Yulan^1,2,4

1. Central Laboratory, Xuanwu Hospital, Capital Medical University, Beijing 100053, China;
2. Beijing Geriatric Medical Research Center, Beijing 100053, China;
3. Center of Alzheimer's Disease, Beijing Institute for Brain Disorders, Beijing 100053, China;
4. Key Laboratory for Neurodegenerative Disease, Ministry of Education, Beijing 100053, China

Received:2015-07-14 Online:2015-10-21 Published:2015-10-20
Contact: 王蓉 E-mail:rong_wang72@aliyun.com
Supported by:
This study was supported by National Natural Science Foundation of China(81472007), Natural Science Foundation of Beijing(7132044).

Abstract

Abstract: Objective To observe the impact of SIRT1 inhibitor (EX527) on the human neuroblastoma cell line SH-SY5Y, and the SH-SY5Y cells were treated with calorie restriction. Methods SH-SY5Y cells were cultured in vitro and divided into the following three groups: normal control group (NC group), calorie restriction group (CR group) and calorie restriction plus SIRT1 inhibitor EX527 group (CR+EX527 group). The morphological changes in the SH-SY5Y cells of each group were observed by light microscopy, and the thiazolyl blue tetrazolium bromide (MTT) metabolic rate and the lactate dehydrogenase (LDH) leakage rate of all groups were measured by MTT working liquid and LDH Cytotoxicity Assay Kit, and the expression of SIRT1 protein in the three groups mentioned above were detected by Western blotting. Results Compared with the normal control (NC) group, cells of CR group were in good shape, including plump cell bodies, good adherence and neurites extension. The MTT metabolic rate of CR group increased (P<0.05), while the LDH leakage rate tended to decrease as compared with NC group. And the expression of SIRT1 protein of CR group and CR+EX527 group were increased obviously (P<0.05) as compared with NC group. Compared with the calorie restriction (CR) group, cells of CR+EX527 group showed poor morphology, including no plump cell bodies, retracted neurites and bad adherence. The MTT metabolic rate of CR+EX527 group decreased (P<0.05), while the LDH leakage rate tended to increase as compared with CR group. And the expression of SIRT1 of CR+EX527 group were decreased slightly as compared with CR group. Conclusion Calorie restriction has a protective effect on nerve cells, and SIRT1 inhibitor EX527 can weaken the neuroprotective effect of calorie restriction in this study, so we preliminarily infer that the protective effect of calorie restriction on SH-SY5Y cells may be mediated by SIRT1.

Key words: calorie restriction, SIRT1 inhibitor, human neuroblastoma cell line SH-SY5Y

CLC Number:

R592

Zhang Jingshuang, Ma Lina, Wang Rong, Zhang Jingyan, Wu Yanchuan, Zhao Zhiwei, Wang Yulan. Effect of EX527 on the protection of SH-SY5Y by calorie restriction[J]. Journal of Capital Medical University, 2015, 36(5): 705-708.

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Effect of EX527 on the protection of SH-SY5Y by calorie restriction

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