Promoter methylation analysis of seven clock genes in the brain of 3xTg mice

doi:10.3969/j.issn.1006-7795.2015.06.008

Journal of Capital Medical University ›› 2015, Vol. 36 ›› Issue (6): 875-881.doi: 10.3969/j.issn.1006-7795.2015.06.008

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Promoter methylation analysis of seven clock genes in the brain of 3xTg mice

Bao Xunjie^1,3, Cai yanning^2,3, Zhu Yanqiu^1,3, Yang Cuicui^1,3, Li Lin^1,3, Zhang Lan^1,3

1. Department of Pharmacology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China;
2. Department of Neurobiology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China;
3. Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China

Received:2015-10-10 Online:2015-12-21 Published:2015-12-18
Supported by:
This study was supported by National Science and Technology Major Project for Essential Drug Research and Development(2015ZX09101016), Beijing Health and Technical High-Level Personal Plan(2014-2-014), New Medical Disciplines Project of Beijing Education Committee(XK100270569).

Abstract

Abstract: Objective To investigate whether abnormal CpG methylation contributes to circadian dysfunction in Alzheimer's disease. Methods We examined the methylation of clock promoters in the mice brain by using a methylation-specific polymerase chain reaction(MSP) assay. Results Methylation was detectable in the cry2;clock and bmal2 promoters in model group, although the frequency is low. However no positive result was found in control group. Conclusion Our results show that the promoter methylation may contribute to changes in circadian rhythms in certain slave oscillators, but not a key point.

Key words: 3xTg mice, promoter methylation, clock genes

CLC Number:

Bao Xunjie, Cai yanning, Zhu Yanqiu, Yang Cuicui, Li Lin, Zhang Lan. Promoter methylation analysis of seven clock genes in the brain of 3xTg mice[J]. Journal of Capital Medical University, 2015, 36(6): 875-881.

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Promoter methylation analysis of seven clock genes in the brain of 3xTg mice

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