Effect of different injection doses of rapamycin on general function changes in miniature pig

doi:10.3969/j.issn.1006-7795.2016.03.002

Journal of Capital Medical University ›› 2016, Vol. 37 ›› Issue (3): 260-263.doi: 10.3969/j.issn.1006-7795.2016.03.002

Previous Articles Next Articles

Effect of different injection doses of rapamycin on general function changes in miniature pig

Zhu Zhao, Pang Baoxing, Wu Xiaoshan, Hu Lei, Hu Liang, Wang Jinsong, Zhang Chunmei, Wang Songling

Molecular Laboratory for Gene Therapy and Tooth Regeneration, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University, Beijing 100050, China

Received:2016-03-29 Online:2016-06-21 Published:2016-06-13
Supported by:
This study was supported by Major State Basic Research Development Program of China (2010CB944801), Beijing Municipality Government Grants (Beijing Scholar Program (PXM2014, PXM 2013 and TJSHG201310025005).

Abstract

Abstract: Objective To evaluate the effect of different injection doses of rapamycin on general function changes in miniature pig (minipig). Methods Twelve minipigs were divided into 3 groups (n=3 per group), which were injected with 0, 0.05, 0.10, 0.15 mg·kg^-1·day^-1 for 7 continuous days separately. Blood samples were obtained every 2 weeks. The assessment of main viscera of minipigs 12 weeks after injection was performed by hematoxylin and eosin (H＆E) staining. Results The numbers of white blood cells, platelets and lymphocyte ratios were dose-dependently decreased at week 2 after injection, and returned to normal range at week 4. However, there was no significant difference in the main viscera of minipigs. Conclusion Seven continuous days injection with rapamycin had no impact on general condition of minipigs.

Key words: rapamycin, dose-dependent, miniature pig

CLC Number:

R979.5

Zhu Zhao, Pang Baoxing, Wu Xiaoshan, Hu Lei, Hu Liang, Wang Jinsong, Zhang Chunmei, Wang Songling. Effect of different injection doses of rapamycin on general function changes in miniature pig[J]. Journal of Capital Medical University, 2016, 37(3): 260-263.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2016.03.002

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2016/V37/I3/260

References

[1] Gingras A C, Raught B, Sonenberg N. Regulation of translation initiation by FRAP/mTOR[J]. Genes Dev, 2001, 15(7): 807-826.
[2] Sarbassov D D, Ali S M, Sabatini D M. Growing roles for the mTOR pathway[J]. Curr Opin Cell Biol, 2005, 17(6): 596-603.
[3] Stepkowski S M, Chen H, Daloze P, et al. Rapamycin, a potent immunosuppressive drug for vascularized heart, kidney, and small bowel transplantation in the rat[J]. Transplantation, 1991, 51(1): 22-26.
[4] Granger D K, Cromwell J W, Chen S C, et al. Prolongation of renal allograft survival in a large animal model by oral rapamycin monotherapy[J]. Transplantation, 1995, 59(2): 183-186.
[5] Harrison D E, Strong R, Sharp Z D, et al. Rapamycin fed late in life extends lifespan in genetically heterogeneous mice[J]. Nature, 2009, 460(7253): 392-395.
[6] Maiese K. Driving neural regeneration through the mammalian target of rapamycin[J]. Neural Regen Res, 2014, 9(15): 1413-1417.
[7] Vogt P K. PI 3-kinase, mTOR, protein synthesis and cancer[J]. Trends Mol Med, 2001, 7(11): 482-484.
[8] Czerninski R, Amornphimoltham P, Patel V, et al. Targeting mammalian target of rapamycin by rapamycin prevents tumor progression in an oral-specific chemical carcinogenesis model[J]. Cancer Prev Res (Phila), 2009, 2(1): 27-36.
[9] Iglesias-Bartolome R, Patel V, Cotrim A, et al. mTOR inhibition prevents epithelial stem cell senescence and protects from radiation-induced mucositis[J]. Cell Stem Cell, 2012, 11(3): 401-414.
[10] Morgan-Bathke M, Harris Z I, Arnett D G, et al. The Rapalogue, CCI-779, improves salivary gland function following radiation[J]. PLoS One, 2014, 9(12): e113183.
[11] 袁进,顾为望. 小型猪作为人类疾病动物模型在生物医学研究中的应用[J]. 动物医学进展,2011,32(2): 108-111.
[12] 单广夷,付成,陈昂,等. 雷帕霉素对膀胱癌5637细胞增殖及细胞周期的影响[J]. 中国医科大学学报,2015,44(3):230-233.
[13] Jimeno A, Rudek M A, Kulesza P, et al. Pharmacodynamic-guided modified continuous reassessment method-based, dose-finding study of rapamycin in adult patients with solid tumors[J]. J Clin Oncol, 2008, 26(25): 4172-4179.
[14] Ireland R. Pediatrics: Sirolimus may stunt growth of kidney-transplanted children[J]. Nat Rev Nephrol, 2011, 7(6): 303.
[15] Kahan B D, Kramer W G. Median effect analysis of efficacy versus adverse effects of immunosuppressants[J]. Clin Pharmacol Ther, 2001, 70(1): 74-81.
[16] Groth C G, Backman L, Morales J M, et al. Sirolimus (rapamycin)-based therapy in human renal transplantation: similar efficacy and different toxicity compared with cyclosporine. Sirolimus European Renal Transplant Study Group[J]. Transplantation, 1999, 67(7): 1036-1042.
[17] Yatscoff R W, Wang P, Chan K, et al. Rapamycin: distribution, pharmacokinetics, and therapeutic range investigations[J]. Ther Drug Monit, 1995, 17(6): 666-671.
[18] 刘杰,杜雨轩,王望,等. 雷帕霉素对野百合碱诱导的大鼠肺动脉高压及肺血管重构的影响 [J]. 首都医科大学学报,2013,34(4):554-558.

Effect of different injection doses of rapamycin on general function changes in miniature pig

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 3

Recommended Articles

Metrics

Comments

[1]	Wang Jieying, Zhu Wei, Lian Shi. Topical rapamycin for the treatment of facial angiofibromas in tuberous sclerosis complex [J]. Journal of Capital Medical University, 2017, 38(5): 654-657.
[2]	LIU Jie, DU Yuxuan, WANG Wang, GUO Huan, WANG Jun, WANG Chen. Effects of rapamycin on monocrotaline-induced pulmonary hypertension and pulmonary arterial remodeling in rats [J]. Journal of Capital Medical University, 2013, 34(4): 554-558.
[3]	ZHANG Ting;WANG Xiao-min. Breakthrough of the 2009 Year of 《Science》 in Life Science [J]. Journal of Capital Medical University, 2010, 31(1): 136-141.