cPKCγ membrane translocation plays roles in Herkinorin postconditioning against ischemia/reperfusion injury in MCAO mice

doi:10.3969/j.issn.1006-7795.2017.03.002

Abstract

Abstract: Objective To evaluate the effects of Herkinorin postconditioning against ischemia/reperfusion injury in middle cerebral artery occlusion (MCAO) mice and the role of conventional protein kinase Cγ (cPKCγ). Methods Healthy adult male C57BL/6 mice were randomly divided into five groups:control group (Naive), sham-operation group (Sham), ischemia for 1 h and reperfusion group (I/R), I/R and DMSO intraperitoneal injection group (I/R+D),I/R and 10 mg/kg Herkinorin intraperitoneal injection group (I/R+H). Neurobehavioral score, Pole test, Wire hang test, Cylinder test and Foot fault test were performed at 24 h and 7 d after reperfusion. Using MCAO induced ischemic stroke mouse model, cerebral infarct volume was evaluated with TTC staining, the cPKCγ membrane translocation levels were detected with Western blotting. Results MCAO induced ischemia/reperfusion injury resulted in increased neurobehavioral scores in mice. Compared with I/R group, neurobehavioral scores of I/R+H group were decreased significantly. Ischemia/reperfusion injury affected sensorimotor function. Pole test, Cylinder test and Foot fault test of I/R+H group were relieved significantly compared with I/R group (P<0.05,n=6). TTC staining showed that infarct volumes in I/R group were 31.44%±5.44% at 24 h and 23.44%±7.95% at 7 d, infarct volumes were decreased in I/R+H group (24 h:17.19%±3.23%,7 d:13.26%±2.71%) (P<0.05,n=6). Western blotting showed cPKCγ membrane translocation levels of ischemic core and peri-infarct regions in MCAO mice cortex were decreased significantly after ischemia for 1 h and reperfusion. Herkinorin alleviated the decrease of cPKCγ membrane translocation levels in cortex peri-infarct region (P<0.05,n=6). Conclusion 10 mg/kg Herkinorin could decrease neurobehavioral score, alleviate Pole test, Cylinder test and Foot fault test evaluation, decrease infarct volumes in MCAO mice. cPKCγ membrane translocation in cortex peri-infarct region may participate molecular mechanism of Herkinorin induced neuroprotection.

Key words: Herkinorin, postconditioning, ischemia/reperfusion, neuroprotection, protein kinases C

CLC Number:

R614.1

Cui Xu, Ji Fang, Shu Luowa, Li Junfa, Pan Chuxiong. cPKCγ membrane translocation plays roles in Herkinorin postconditioning against ischemia/reperfusion injury in MCAO mice[J]. Journal of Capital Medical University, 2017, 38(3): 336-341.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.03.002

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I3/336

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