Generation of oligodendrocyte progenitor cells from human peripheral blood mononuclear cells

doi:10.3969/j.issn.1006-7795.2017.04.013

Abstract

Abstract: Objective To generate oligodendrocyte progenitor cells (OPCs) from peripheral blood mononuclear cells by episomal vectors.Methods Peripheral blood of a donor was collected by venipuncture and then mononuclear cells (MNCs) were isolated by density-based centrifugal separation. After a short period of expansion, the isolated MNCs were transfected with three plasmids expressing OCT4, SOX2, KLF4, C-MYC, LIN28, NANOG, and cultured in chemical defined medium with small molecules. Results Sixty days later, the O4 oligodendrocyte progenitor cells appeared and could be expanded more than 60 passages. Conclusion The peripheral blood MNCs can be converted to oligodendrocyte progenitor cells by non-integrative plasmid vectors.

Key words: peripheral blood mononuclear cells, cell reprogramming, oligodendrocyte progenitor cells

CLC Number:

R329.2

Tang Xihe, Li Mo, Wang Shuyan, Li Pengyan, Zhang Y. Alex, Chen Zhiguo, Chen Hui. Generation of oligodendrocyte progenitor cells from human peripheral blood mononuclear cells[J]. Journal of Capital Medical University, 2017, 38(4): 553-558.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.04.013

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I4/553

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