Study on Wenshen Yangxue decoction to improve oocyte quality and embryonic development potential of old female rats

doi:10.3969/j.issn.1006-7795.2018.06.005

Journal of Capital Medical University ›› 2018, Vol. 39 ›› Issue (6): 815-820.doi: 10.3969/j.issn.1006-7795.2018.06.005

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Study on Wenshen Yangxue decoction to improve oocyte quality and embryonic development potential of old female rats

Xin Mingwei¹, He Junqin¹, Jia Zhaoxia², Wu Ying¹, Zhang Ying¹, Yang Wei¹, Yin Xiaodan¹

1. Department of Traditional Chinese Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China;
2. Information and Statistics Department, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China

Received:2018-09-20 Online:2018-11-21 Published:2018-12-19
Supported by:
This study was supported by Natural Science Foundation of Beijing(7152069), Capital Health Research and Development of Special Funding(2018-1-2122),Cooperation between Preclinical and Clinical Medicine of Capital Medical University(16JL84).

Abstract

Abstract: Objective To explore the mechanism of Wenshen Yangxue Decoction in improving oocyte quality and embryo development potential in older female rats.Methods Eight-month-old ICR mice were divided into control group, pregnant mare serum gonadotropin (PMSG) group, Wenshen decoction, Yangxue decoction and Wenshen Yangxue decoction according to random number table method. The fertilization rate and cleavage rate of every group after treatment were recorded, and the expression of mitochondria in oocyte and fertilized eggs were examined. Results The fertilization rate and cleavage rate of Wenshen Yangxue decoction, Wenshen decoction and Yangxue decoction were significantly higher than those of the control group and PMSG group (P<0.05). The fertilization rate and cleavage rate of Wenshe Yangxue decoction were the highest (P<0.05). The 2 cell rate, 4 cell rate, 8 cell rate, morula rate and blastocyst rate of Wenshen Yangxue decoction were higher than in the PMSG group (P<0.05). The mitochondrial DNA copy number in oocytes and fertilized eggs of Wenshen Yangxue decoction was the highest of the five groups, which was statistically significant compared with the control group(P<0.05). Conclusion Wenshen Yangxue decoction may improve oocyte quality and embryo development potential by regulating oocyte mitochondrial DNA copy number.

Key words: Wenshen Yangxue decoction, old female rats, oocyte quality, mitochondrial

CLC Number:

R285.5

Xin Mingwei, He Junqin, Jia Zhaoxia, Wu Ying, Zhang Ying, Yang Wei, Yin Xiaodan. Study on Wenshen Yangxue decoction to improve oocyte quality and embryonic development potential of old female rats[J]. Journal of Capital Medical University, 2018, 39(6): 815-820.

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References

[1] 辛明蔚,张秋霞,赵晖,等. 温肾养血方对无排卵大鼠抑制素-激活素-卵泡抑素系统的影响[J].中国中西医结合杂志,2016,36(12):1491-1495.
[2] 辛明蔚,张秋霞,赵晖,等. 温肾养血方对无排卵模型大鼠卵巢质量和形态的影响[J].安徽中医药大学学报,2015,34(3):68-71.
[3] 李秦,朱德生,刘玉林,等. 不同激素对哺乳动物超数排卵作用的研究[J]. 实验动物科学与管理,1997,14(3):1-6.
[4] 闫松.黄帝内经[M].北京:线装书局,2010:2.
[5] (宋)陈自明. 妇人大全良方[M]. 太原:山西科学技术出版社, 2006:12.
[6] 田海丽,李蓉. 卵母细胞线粒体质量改善的相关研究进展[J]. 现代妇产科进展,2018,27(3):225-231.
[7] 李红真,乔杰. 氧化应激与卵巢老化[J]. 中国实用妇科与产科杂志,2015, 31(8):774-778.
[8] 徐海琦,林辉荣,吴春丽,等. 哺乳动物卵母细胞退化机制[J]. 四川生理科学杂志,2012,34(2):82-85.
[9] López-Otín C,Galluzzi L,Freije J M P,et al. Metabolic control of longevity[J].Cell,2016,166(4):802-821.
[10] Sutovsky P,Moreno R D,Ramalho-Santos J,et al. Ubiquitin tag for sperm mitochondria[J]. Nature,1999,402(6760):371-372.
[11] 贾振伟,张显华.线粒体在调控卵母细胞成熟和发育中的作用[J].中国畜牧杂志,2016,52(7):96-100.
[12] Diez-Juan A,Rubio C,Marin C,et al.Mitochondrial DNA content as a viability score in human euploid embryos:less is better[J]. Fertil Steril,2015,104(3):534-541.
[13] 王元芬,郝建秀,王辉,等. 线粒体功能在卵母细胞成熟中的作用综述[J]. 解放军医学院学报,2017,38(9):987-989.
[14] EI Shourbagy S H,Spikings E C,Freitas M,et al. Mitochondriadirectly influence fertilisation outcome in the pig[J]. Reproduction,2006,131(2):233-245.
[15] 侯海燕,郁琦.线粒体功能障碍与卵巢衰老[J].国际妇产科学杂志,2017,44(4):450-454.
[16] Van Blerkom J. Mitochondrial function in the human oocyte and embryo and their role in developmental competence[J]. Mitochondrion,2011,11(5):797-813.

Study on Wenshen Yangxue decoction to improve oocyte quality and embryonic development potential of old female rats

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