Relationship between the expression of heat shock factor 1 and mitochondrial damage in aging myocardium

doi:10.3969/j.issn.1006-7795.2018.02.016

Abstract

Abstract: Objective To explore the relationship between mitochondrial damage and the expression of heat shock factor 1 (HSF1) in aging myocardium.Methods Four-month-old and 24-month-old C57BL/6 mice were used as the young and aging mices. The changes of the morphology in mitochondria were observed by transmission electron microscopy in the myocardial tissue.The concentration of ATP and the production of reactive oxygen species (ROS) in aging myocardium and H9C2 cells were detected by related kits.JC-1 was used to detect the change of membrane potential in H9C2 cells.The expression of HSF1 was detected by Western blotting. Results In the aging myocardium, the morphology of mitochondria was changed and the concentration of ATP was decreased(1.253±0.059 vs 0.479±0.122, P=0.012), the production of ROS was increased(10.853±0.096 vs 1.550±0.233, P=0.025), and the expression of HSF1 was also increased in the aging myocardium (0.980±0.112 vs 1.979±0.175, P=0.013). The expression of HSF1 in aging myocardium was negatively correlated with the content of ATP (r=-0.977, P=0.005) and positively correlated with the content of ROS (r=0.934, P=0.020). After treated with H₂O₂, the mitochondrial membrane potential of cardiomyocytes decreased, the content of ATP decreased and the content of ROS increased,the expression of HSF1 was increased in the cardiomyocytes(P<0.05).Conclusion During the aging process,the mitochondria is damaged and the dysfunction of mitochondria was negatively correlated with the expression of HSF1 in aging myocardium.

Key words: heat shock factor, aging myocardium, mitochondrial damage

CLC Number:

R363

Wu Linguo, Liu Dan, Wu Ye, Ma Xinliang, Liu Huirong. Relationship between the expression of heat shock factor 1 and mitochondrial damage in aging myocardium[J]. Journal of Capital Medical University, 2018, 39(2): 236-242.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2018.02.016

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2018/V39/I2/236

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