Establishment and comparison of two hypoxic pulmonary hypertension models in mice

doi:10.3969/j.issn.1006-7795.2018.04.016

Abstract

Abstract: Objective To compare the similarities and differences between the model of hypoxia and hypoxia combined SU5416 modeling method, explore whether hypoxia combined with SU5416 can establish a more significant model of pulmonary hypertension in mice. Methods Totally 15 male C57BL/6 mice were randomly divided into 3 groups:normoxia, hypoxia and hypoxia+SU5416. After four weeks experiment, body weight (BW), right ventricular systolic pressure (RVSP), right ventricular hypertrophy index (RVHI), right ventricular/body weight (RV/BW), lung morphological features and medial thickness (MT%) were assessed. Results After 4 weeks, the body weight of the mice in normoxia was significantly higher than that in hypoxia and hypoxia+SU5416, whereas the RVSP, RVHI, RV/BW and MT% of the mice in hypoxia and hypoxia+SU5416 were higher than that in normoxia. The RVSP, RVHI and MT% of the hypoxia+SU5416 were higher than those in hypoxia, while the RV/BW of hypoxia+SU5416 was not significantly different from that of hypoxia. Conclusion These results suggested that hypoxia combined with SU5416 can establish a more significant pulmonary hypertension model than hypoxia alone in mice.

Key words: hypoxia, hypoxia combined with SU5416, pulmonary arterial hypertension

CLC Number:

R363

Gao Yang, Liu Jie, Wang Lei, Zhang Wei, Liu Yafei, Qi Xianmei, Wang Wang. Establishment and comparison of two hypoxic pulmonary hypertension models in mice[J]. Journal of Capital Medical University, 2018, 39(4): 563-568.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2018.04.016

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2018/V39/I4/563

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