Effect of lipopolysaccharide on the expression of α-synuclein in the intestinal tract

doi:10.3969/j.issn.1006-7795.2018.04.015

Journal of Capital Medical University ›› 2018, Vol. 39 ›› Issue (4): 557-562.doi: 10.3969/j.issn.1006-7795.2018.04.015

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Effect of lipopolysaccharide on the expression of α-synuclein in the intestinal tract

Liu Mengru^1,2, Gong Xiaoli^2,3, Wang Le^1,2, Liu Yang^1,2, Zhang Ting^1,2, Wang Xiaomin^1,2

1. Department of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China;
2. Key Laboratory of Neurodegenerative Disorders of the Ministry of Education, Capital Medical University, Beijing 100069, China;
3. Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Received:2018-05-21 Online:2018-07-21 Published:2018-07-21
Supported by:
This study was supported by National Key Research and Development Program (2016YFC1306300), Major Program of the National Natural Science Foundation of China (81527901), Natural Science Foundation of Beijing (7162019),National Major Research and Development Program of Beijing Municipal Science & Technology Commission (Z161100002616007).

Abstract

Abstract: Objective Intraperitoneal injection of lipopolysaccharide (LPS) was used to construct Parkinson's disease (PD) mouse model. The protein level of α-synuclein (α-syn) and intestinal inflammation were detected in intestinal tissue of PD mice, to explore the expression of key pathogenic protein in the intestinal tract of early PD. Methods Male C57BL/6J mice were divided into control group and model group, mice received intraperitoneal injection of LPS or 0.9% saline. The mice model was characterized by detecting the level of inflammatory factor tumor necrosis factor-α(TNF-α) in blood and body weight. The protein level of α-synuclein(α-syn) and inflammasome NOD-like receptor protein 3 (NLRP3) in intestine were detected by Western blotting, the expression of TNF-α was measured by reverse transcription-polymerase chain reaction (RT-qPCR). Results In the acute phase, the expression of α-syn, NLRP3 and TNF-α were increased after LPS injection, the α-syn and NLRP3 returned to baseline levels at one month after LPS administration. But the expression of TNF-α was still at a high level. Conclusion LPS promoted the expression of α-syn in the intestine, which was accompanied by inflammasome activation and TNF-α expression. However, the high level of TNF-α is likely to lead to the progressive increase of α-syn in the chronic period of PD model induced by LPS.

Key words: α-synuclein, inflammation, intestine, Parkinson's disease

CLC Number:

R741.02

Liu Mengru, Gong Xiaoli, Wang Le, Liu Yang, Zhang Ting, Wang Xiaomin. Effect of lipopolysaccharide on the expression of α-synuclein in the intestinal tract[J]. Journal of Capital Medical University, 2018, 39(4): 557-562.

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Effect of lipopolysaccharide on the expression of α-synuclein in the intestinal tract

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