Effect of chronicunpredictable stress on neurotransmitter and hypothalamic-pituitary-adrenal axis in rats

doi:10.3969/j.issn.1006-7795.2015.01.024

Abstract

Abstract: Objective To investigate the effect of chronic unpredictable stress (CUS) on neurotransmitter and hypothalamic-pituitary-adrenal axis(HPA)in rats. Methods Totally 16 male SD rats were randomly divided into normal group and model group. The model group was established by chronic unpredictable stress. The weights of rats were examined every day.The behavior changes of rats were observed by the sucrose preference test. The contents of dopamine(DA), 5-hydroxeytryptamine(5-HT)inhippocampus were measured by High performance liquid-electrochemical method. And the secretion of glutamic acid(Glu), γ-aminobutyric acid(γ-GABA) in hippocampus were determined by the method of high-effective liquid chromatography fluorescence.The concentrations of corticotropin-releasing hormone(CRH), adrenocorticotropic hormone (ACTH), corticosterone (CORT) in plasma and hypothalamus were tested by Radioimmunoassay(RIA)method. Results Compared with the normal group, the model group's weight was significantly reduced. The consumption of sucrose was decreased in model group. The contents of DA, γ-GABA in hippocampus was significantly reduced in model group, and the 5-HT, Glu had the trend of increase.The adrenocorticotropic hormone(ACTH) in hypothalamus was significantly decreased and the plasma's corticosterone(CORT) was significantly increased in model group.Conclusion The chronic unpredictable stress (CUS) could induce the changes of animal behavior and abnormalities of neurotransmitter and hypothalamic pituitary adrenal (HPA)axis.

Key words: chronic-unpredictable-stress, behavior, neurotransmitter, hypothalamic-pituitary-adrenal axis

CLC Number:

R363

Yang Yilong, Yao Xiaoquan, Wang Yali, Wang Haizheng, Ouyang Junyao, Liu Sisi, Wang Lei, Zhang Qiuxia, Zhao Hui. Effect of chronicunpredictable stress on neurotransmitter and hypothalamic-pituitary-adrenal axis in rats[J]. Journal of Capital Medical University, 2015, 36(1): 127-131.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2015.01.024

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2015/V36/I1/127

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