Protective effect of postconditioning on myocardial ischemic reperfusion injury in aged rats

doi:10.3969/j.issn.1006-7795.2015.01.025

Abstract

Abstract: Objective To study the effect of ischemic postconditioning(IPC) on cardiomyocyte apoptosis of aged rats with myocardial ischemia reperfusion. Methods The aged(22-24 months) male Wister rats were randomly divided into 3 groups(30 animals in each group): aged sham group, aged ischemic reperfusion group and aged ischemic postconditioning group. Rats in the ischemic reperfusion groups were subjected to 40 minutes of myocardial ischemia by ligating the left anterior descending coronary artery(LAD), followed by a release of the ligature and 6 h of reperfusion. Ischemic postconditionging groups were treated for 40 minutes ischemia, followed by three 10 s cycles of reperfusion and re-occlusion applied at the onset of 6 h of reperfusion. A silk suture around the LAD was not ligated in the sham-operation groups. Myocardial apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL) staining. The levels of superoxide dismutase(SOD) and malonaldehyde(MDA) in serum were detected by colorimetry. Results The aged rat hearts had markedly decreased cardiomyocyte apoptosis in the IPC compared with the IRG[AI(%):45.51±8.81 vs 53.99±10.54, P<0.01]. The serum levels of SOD were higher in the IPC than that in the IRG(277.70±29.55 vs 303.72±25.25, P<0.05), while lower levels of MDA were found in the IPC(25.02±2.35 vs 22.54±2.64, P<0.05). Conclusion The IPC can inhibit cardiomyocyte apoptosis induced by the ischemic reperfusion injury, probably results from the increased antioxidant efficiency while decreased oxidative damage. The IP has protective effects on the acute aged ischemic reperfusion myocardium.

Key words: apoptosis, ischemic postconditioning, myocardial reperfusion injury, aging, cardioprotection

CLC Number:

R743.31

Wang Mengran, Zhang Jian, Liang Yanhong, Chen Yanrong, Li Min, Xiao Yao, Xiu Hui. Protective effect of postconditioning on myocardial ischemic reperfusion injury in aged rats[J]. Journal of Capital Medical University, 2015, 36(1): 132-136.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2015.01.025

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2015/V36/I1/132

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