Investigation on the inhibition of Sitagliptin by osmotic pump on CD26/DPPIV activity

doi:10.3969/j.issn.1006-7795.2019.03.018

Journal of Capital Medical University ›› 2019, Vol. 40 ›› Issue (3): 424-428.doi: 10.3969/j.issn.1006-7795.2019.03.018

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Investigation on the inhibition of Sitagliptin by osmotic pump on CD26/DPPIV activity

Xue Xingkui¹, Han Xinhua², Cai Siqi^1,3, Chen Xiaoling¹, Melody Y. Zeng¹

1. Central Lab, The People's Hospital of Longhua Shenzhen, Shenzhen 518109, Guangdong Province, China;
2. Department of Endocrinology, Shenzhen Shekou People's Hospital, Shenzhen 518109, Guangdong Province, China;
3. Department of Laboratory Medicine and Biotechnology, Graduate School, Southern Medical University, Guangzhou 501505, China

Received:2019-02-15 Online:2019-05-21 Published:2019-06-13
Supported by:
This study was supported by National Natural Sciences Foundation of China (31650110474),Shenzhen Science,Technology and Innovation Commission (JCYJ20160426094752965),Shenzhen Health Research Project (201601059),Funds for Innovation of Science and Technology in Longhua District,Shenzhen (20160831A1030180,20160831A0410020).

Abstract

Abstract: Objective To investigate the effect of Sitagliptin on mice with subcutaneous osmotic pump. Methods Osmotic pump carrying with Sitaglipin was implanted into mice subcutaneously, and the plasma concentration of sitagliptin was measured with HPLC-MS. The skin inflammation reaction was evaluated with pathological slides. DPPIV enzyme activity was measured with ELISA. Furthermore, the CD26 expression of spleen T cell was detected with flow cytometry and T cell migration was determined with transwell system.Results The duration of plasm Sitagliptin in mice subject to instant shot was shorter while those delivered by osmotic pump was observed at 24h post implantation of osmotic pump. Inhibition of DPPIV activity, as measured by ELISA, was confirmed at 24 hours after pump implantation, highlighting the utility of this method as a longer term model for DPPIV inhibition in mice. T cell migration was stably inhibited after application of Sitagliptin with osmotic pump. Conclusion In vivo Sitagliptin administration by an osmotic pump offers an effective approach for longer-term and stable DPPIV inhibition in mice and avoids the necessity for multiple dosing of the inhibitor, making study on function of CD26/DPPIV more effective.

Key words: CD26/DPPIV, Sitagliptin, osmotic pump, T cells

CLC Number:

R363

Xue Xingkui, Han Xinhua, Cai Siqi, Chen Xiaoling, Melody Y. Zeng. Investigation on the inhibition of Sitagliptin by osmotic pump on CD26/DPPIV activity[J]. Journal of Capital Medical University, 2019, 40(3): 424-428.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2019.03.018

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2019/V40/I3/424

References

[1] Ohnuma K, Hatano R, Komiya E, et al. A novel role for CD26/dipeptidyl peptidase IV as a therapeutic target[J]. Front Biosci (Landmark Ed), 2018, 23:1754-1779.
[2] Farag S S, Srivastava S, Messina-Graham S, et al. In vivo DPP-4 inhibition to enhance engraftment of single-unit cord blood transplants in adults with hematological malignancies[J]. Stem Cells Dev, 2013, 22(7):1007-1015.
[3] 李颖. 间充质干细胞条件培养基对糖尿病肾病大鼠肾脏的保护作用[J]. 中华老年多器官疾病杂志, 2017, 2(16):140-143.
[4] Wang M T, Lin S C, Tang P L, et al. The impact of DPP-4 inhibitors on long-term survival among diabetic patients after first acute myocardial infarction[J]. Cardiovasc Diabetol, 2017, 16(1):89.
[5] Cordero O J, Varela-Calvino R, Lopez-Gonzalez T, et al. Anti-CD26 autoantibodies are involved in rheumatoid arthritis and show potential clinical interest[J]. Clin Biochem, 2017, 50(16-17):903-910.
[6] Fernandez-Paredes L, Casrouge A, Decalf J, et al. Multimarker risk stratification approach at multiple sclerosis onset[J]. Clin Immunol, 2017, 181:43-50.
[7] Papazafiropoulou A K, Papanas N, Trikkalinou A, et al. The oral dipeptidyl-peptidase-4 inhibitor sitagliptin Increases circulating levels of stromal-derived factor-1 alpha[J]. Exp Clin Endocrinol Diabetes, 2018, 126(6):367-370.
[8] Farag S S, Nelson R, Cairo M S, et al. High-dose sitagliptin for systemic inhibition of dipeptidylpeptidase-4 to enhance engraftment of single cord umbilical cord blood transplantation[J]. Oncotarget, 2017, 8(66):110350-110357.
[9] Waget A, Cabou C, Masseboeuf M, et al. Physiological and pharmacological mechanisms through which the DPP-4 inhibitor sitagliptin regulates glycemia in mice[J]. Endocrinology, 2011, 152(8):3018-3029.
[10] 刘珊珊. LC-MS/MS法分析人血浆中西格列汀浓度及其应用研究[J]. 天津医药, 2018, 10(46):1096-1101.
[11] Balakrishnan A, Gloude N, Sasik R, et al. Proinflammatory dual receptor T cells in chronic graft-versus-host disease[J]. Biol Blood Marrow Transplant, 2017, 23(11):1852-1860.
[12] 范一帆,齐丹,刘佳馨,等.鞘磷脂合酶抑制剂对血管紧张素Ⅱ诱导的高血压小鼠心肌纤维化的影响[J].首都医科大学学报,2016,37(6):731-735.

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Investigation on the inhibition of Sitagliptin by osmotic pump on CD26/DPPIV activity

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