Effect of Qingdu Decoction on autophagy of intestinal epithelial cells in rats with severe liver injury

doi:10.3969/j.issn.1006-7795.2019.03.019

Abstract

Abstract: Objective To observe the effects of Qingdu Decoction (QDD) on endotoxin, interleukin-17 (IL-17), interleukin-23 (IL-23) of serum, autophagy-related proteins LC3 and Beclin-1 in experimental rats with severe liver injury. Methods A rat model of severe liver injury induced by thioacetamide (TAA) was established. SPF Wistar male rats were randomly divided into normal group, model group, lactulose(LA) group and QDD group. Except the normal group, the other groups were given 12 mg/kg TAA daily for 12 weeks. At the end of the 8th week, LA group and QDD group were treated with lactulose 1.6 g/kg, Qingdu decoction 5.95 g/kg daily. After 4-week intervention, HE staining was used to observe the pathological changes of liver and intestinal tissues. Transmission electron microscopy was used to observe autophagosomes in intestinal epithelial cells. IL-17, IL-23 and endotoxin levels were measured by enzyme-linked immuno sorbent assay. The expression levels of autophagy-related proteins LC3 and Beclin-1 in intestinal tissues were detected by Western Blot (WB).Results Compared with the normal group, the liver and intestinal tissue of the model group was inflamed, less autophagosomes, and the concentrations of endotoxin, IL-17 and IL-23 were significantly increased, and the contents of LC3 protein and Beclin-1 were significantly decreased (P<0.01). After the intervention of the Qingdu decoction, the inflammatory infiltration was significantly relieved, autophagosomes have increased significantly, the concentrations of endotoxin, IL-17 and IL-23 were significantly decreased, and the contents of LC3Ⅱ/LC3I and Beclin-1 were significantly increased (P<0.01). Conclusion Qingdu Decoction can reduce intestinal damage, reduce the concentration of endotoxin, IL-17 and IL-23, and increase the number of autophagosomes and the content of LC3, Beclin-1 protein in rats with liver injury induced by TAA. It suggests that Qingdu Decoction may alleviate liver damage in rats with severe liver injury by promoting autophagy of intestinal epithelial cells and reducing endotoxin production and the release of inflammatory factors.

Key words: liver injury, endotoxin, autophagy, thioacetamide

CLC Number:

R289
R575. 1

Zhao Mengpei, Li Aihong, Cui Ying, Che Niancong, Zhang Qiuyun, Du Yuqiong, Fu Xiuwen, Gao Lianyin. Effect of Qingdu Decoction on autophagy of intestinal epithelial cells in rats with severe liver injury[J]. Journal of Capital Medical University, 2019, 40(3): 429-433.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2019.03.019

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2019/V40/I3/429

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