Mechanism of glucagon-like peptide 1 regulating the activity of brown adipose tissue in diabetic db mice by angiopoietin-like protein 4

doi:10.3969/j.issn.1006-7795.2020.02.012

Abstract

Abstract: Objective To explore glucagon-like peptide 1 (GLP-1) whether and how to regulate the function of brown adipose tissue (BAT) though angiopoietin-like protein 4 (ANGPTL4). Methods Mice were randomized and controlled to receive subcutaneous pump intervention for 6 weeks. Before sampling, glucose tolerance test and pet-CT were conducted. Fasting blood, brown fat and liver were taken for cholesterol and triglyceride quantification, FPLC, qPCR, Western blotting, and transcriptometric sequencing, etc. Results GLP-1 analogue exendin-4 (Ex-4) improved abnormal glucose tolerance in diabetic db/db mice and reduced triglyceride levels in peripheral blood and BAT. Ex-4 promoted the expression of UCP-1 in BAT, and pet-CT indicated increased activity; Ex-4 promotes the expression of ANGPTL4 in BAT; the deletion of ANGPTL4 decreased the activity of BAT. Conclusion GLP-1 promoted BAT activity accompanied by increased expression level of ANGPTL4, and the deficiency of ANGPTL4 lowered the activity and function of BAT, which may become a therapeutic target for the regulation of BAT activity.

Key words: glucagon-like peptide 1, angiopoietin-like protein 4, brown adipose tissue, activity, type 2 diabetes mellitus

CLC Number:

R589.2

Yan Cen, Luo Xiaomin, Feng Yingmei. Mechanism of glucagon-like peptide 1 regulating the activity of brown adipose tissue in diabetic db mice by angiopoietin-like protein 4[J]. Journal of Capital Medical University, 2020, 41(2): 217-224.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2020.02.012

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2020/V41/I2/217

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