Comparison of throat swab and sputum specimens for viral nucleic acid detection in 31 cases of 2019-nCoV infection

doi:10.3969/j.issn.1006-7795.2020.03.003

Journal of Capital Medical University ›› 2020, Vol. 41 ›› Issue (3): 336-339.doi: 10.3969/j.issn.1006-7795.2020.03.003

• Progress in Diagnosis, Treatment and Prognosis of COVID-19 • Previous Articles Next Articles

Comparison of throat swab and sputum specimens for viral nucleic acid detection in 31 cases of 2019-nCoV infection

Guo Jingjing¹, Fang Qian¹, Liu Yanan¹, Qu Pei¹, Ma Xueying¹, Wang Shuang¹, Fang Wei¹, Zhou Ru¹, Zhang Xiang¹, Tang Yunxia², Yang Siyuan², Yu Fengting², Wang Yajie¹

1. Laboratory Department, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China;
2 Laboratory of Infection Emergency Center, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China

Received:2020-03-20 Online:2020-06-21 Published:2020-06-17
Supported by:
This study was supported by Beijing Hospital Management Center COVID-19 Science and Technology Project(COVID-19-BHA05), COVID-19 Science and Technology Prevention and Control Research First Batch of Emergency Projects of Beijing Municipal Commission of Science and Technology (Z201100005420022, Z201100005420012).

Abstract

Abstract: Objective To comparatively analyze the detection efficiency of viral nucleic acid in throat swab and sputum sample of 2019-novel coronavirus(2019-nCoV) cases. Methods Ribonucleoprotein (RNP) gene in human epithelial cells, viral ORF1ab genes and N genes were tested and compared with real-time PCR in throat swabs and sputum specimens from 31 confirmed cases of 2019-nCoV. Results In the throat swabs and sputum samples of 31 cases, the RNP gene of human epithelial cells showed obvious and typical amplification signal curves. In the detection of viral ORF1ab gene and N gene, the amplification signal of sputum samples was stronger than that of throat swab, and the cycle threshold (Ct) value of amplification curve was lower than that of throat swab, especially in case 9 and 16 were single gene positive, the pharyngeal swabs of the 9th and 16th patients were single gene positive, and the 17th patient was double gene positive, and the rest of the 28 patients were negative. Sputum specimens collected at the same time showed that all the 18 patients were single gene positive and 13 were double gene positive. Conclusion In the viral nucleic acid testing of 2019-nCoV laboratory detection, the virus content in sputum specimens was higher than that in throat swab specimens, and the detection efficiency was higher than that in throat swab specimens.

Key words: new coronavirus (2019-nCOV), sputum specimen, throat swab, viral nucleic acid test

CLC Number:

R563.1

Guo Jingjing, Fang Qian, Liu Yanan, Qu Pei, Ma Xueying, Wang Shuang, Fang Wei, Zhou Ru, Zhang Xiang, Tang Yunxia, Yang Siyuan, Yu Fengting, Wang Yajie. Comparison of throat swab and sputum specimens for viral nucleic acid detection in 31 cases of 2019-nCoV infection[J]. Journal of Capital Medical University, 2020, 41(3): 336-339.

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Comparison of throat swab and sputum specimens for viral nucleic acid detection in 31 cases of 2019-nCoV infection

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